Minimum inhibitory concentrations of commonly used antibiotics against Helicobacter Pylori: A multicenter study in South China

• Published in: PloS one 2021-09, Vol.16 (9), p.e0256225-e0256225
• Main Authors: Huang, Xueping, Liu, Yuan, Lin, Zhihui, Wu, Baihe, Nong, Gaohui, Chen, Yushan, Lu, Yuping, Ji, Xinhua, Zhou, Xiang, Suo, Biao, Chen, Qiuzhao, Wei, Jinqi
• Format: Article
• Language: English
• Subjects: Adolescent; Adult; Aged; Amoxicillin; Amoxicillin - pharmacology; Anti-Bacterial Agents - pharmacology; Antibiotics; Biology and Life Sciences; Biopsy; Biotechnology industry; Breakpoints; Care and treatment; China; Chinese medicine; Clarithromycin; Clarithromycin - pharmacology; Complications and side effects; Dose-Response Relationship, Drug; Drug resistance; Drug resistance in microorganisms; Drug Resistance, Bacterial; Endoscopy; Evaluation; Female; Furazolidone; Furazolidone - pharmacology; Gastroenterology; Helicobacter; Helicobacter Infections - drug therapy; Helicobacter Infections - microbiology; Helicobacter Infections - pathology; Helicobacter pylori; Helicobacter pylori - drug effects; Helicobacter pylori - isolation & purification; Hospitals; Humans; Levofloxacin; Levofloxacin - pharmacology; Male; Medicine and Health Sciences; Metronidazole; Microbial Sensitivity Tests; Middle Aged; Patient outcomes; Patients; People and Places; Tetracycline - pharmacology; Ulcers; Young Adult
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0256225

To determine the minimum inhibitory concentrations (MICs) of commonly used antibiotics against Helicobacter Pylori (H. pylori) in South China and compare their resistance rates by using EUCAST breakpoints and other breakpoints. Patients who had not previously received H. pylori treatment in clinical centers in South China were enrolled in this study from 2017 to 2020. Gastric biopsies were obtained for H. pylori culture. The MICs of amoxicillin (AMX), clarithromycin (CLA), metronidazole (MTZ), levofloxacin (LEV), tetracycline (TET) and furazolidone (FZD) were tested by broth microdilution method and assessed by two different breakpoints. ATCC43504 standard strain served as a control. A total of 208 H. pylori strains were isolated from patients' biopsy samples. The MICs of AMX, CLA, MTZ, LEV, TET and FZD for H. pylori were 0.0156-256mg/L (MIC50 0.125mg/L, MIC90 4mg/L), 0.0156- >256 mg/L (MIC50 0.0312mg/L, MIC90 64mg/L), 0.0156- >256mg/L (MIC50 8mg/L, MIC90 256mg/L), 0.0156-256mg/L (MIC50 0.25mg/L, MIC90 16mg/L), 0.0156-256mg/L (MIC50 0.0625mg/L, MIC90 4mg/L), and 0.0156- >256mg/L (MIC50 0.0312mg/L, MIC90 2mg/L), respectively. The MICs of AMX, CLA, MTZ, LEV, TET and FZD for ATCC43504 strain were 0.25mg/L, 0.0625mg/L, 64mg/L, 0.5mg/L, 1mg/L and 0.25mg/L, respectively. The resistance rate of FZD was 11.05%. The overall resistance rates according to EUCAST breakpoints and other breakpoints were 57.21% and 14.90% for AMX (p