The effect of sodium carboxymethyl starch with high degree of substitution on defecation

Sodium carboxymethyl starch (CMS-Na), a kind of food additive with high degree of substitution, is also known as a prebiotic. The aim of this study was to determine the effect of CMS-Na on defecation. Constipated mouse model was prepared by loperamide. Normal rats were also used in the study. Short-...

Full description

Saved in:
Bibliographic Details
Published in:PloS one 2021-09, Vol.16 (9), p.e0257012-e0257012
Main Authors: Lu, Wu-dang, Wu, Man-li, Zhang, Jun-xia, Huang, Ting-ting, Du, Shuai-shuai, Cao, Yong-xiao
Format: Article
Language:English
Subjects:
Acetic acid
Animals
Biodiversity
Biology and Life Sciences
Care and treatment
Colon
Constipation
Defecation
Fatty acids
Feces
Flora
Food additives
Gas chromatography
Gene sequencing
Health aspects
Intestinal microflora
Intestine
Lactobacillus
Medicine and Health Sciences
Mice
Nitrogen
Pharmaceuticals
Physical Sciences
Prebiotics
Propionic acid
Relative abundance
Research and Analysis Methods
rRNA 16S
Small intestine
Sodium
Starch
Statistical analysis
Substitutes
Tryptophan
Tryptophan hydroxylase
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Sodium carboxymethyl starch (CMS-Na), a kind of food additive with high degree of substitution, is also known as a prebiotic. The aim of this study was to determine the effect of CMS-Na on defecation. Constipated mouse model was prepared by loperamide. Normal rats were also used in the study. Short-chain fatty acids in rat feces were detected by gas chromatography. The bacterial communities in rat feces were identified by 16S rDNA gene sequencing. 5-hydroxytryptamine (5-HT) and tryptophan hydroxylase 1 (Tph1) were measured by ELISA. The results showed that CMS-Na increased the fecal granule counts and intestinal propulsion rate in constipated mice. The contents of water, acetic acid, propionic acid and n-butyrate in feces, Tph1 in colon and 5-HT in serum of rats were increased. In addition, CMS-Na shortened the colonic transport time in rats. The 16S rDNA gene sequencing results indicated that CMS-Na increased the relative abundance of Alloprevotella and decreased the proportion of Lactobacillus. However, the biodiversity of the normal intestinal flora was not altered. In conclusion, CMS-Na can promote defecation in constipated mice. The mechanism may be related to the regulation of Alloprevotella and Lactobacillus in colon, the increase of short-chain fatty acids, and the promotion of the synthesis of Tph1 and 5-HT.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0257012