Mining nematode protein secretomes to explain lifestyle and host specificity

• Published in: PLoS neglected tropical diseases 2021-09, Vol.15 (9), p.e0009828-e0009828
• Main Authors: Tritten, Lucienne, Ballesteros, Cristina, Beech, Robin, Geary, Timothy G, Moreno, Yovany
• Format: Article
• Language: English
• Subjects: Aldolase; Analysis; Animal diseases; Animal parasites; Animals; Antigens; Biology and Life Sciences; Caenorhabditis elegans; Care and treatment; Cytokines; Datasets; Diagnosis; Disease transmission; Ecology and Environmental Sciences; Engineering and Technology; Engineering research; Enzymes; Female; Genetic aspects; Glycolysis; Helminth Proteins - classification; Helminth Proteins - metabolism; Host preferences; Host Specificity; Humans; Identification and classification; Immune system; Immunity; Infections; Larvae; Life Cycle Stages; Life cycles; Lifestyles; Male; Medicine and Health Sciences; Molecules; Nematoda; Nematoda - physiology; Nematodes; Parasites; Parasitic diseases; Parasitism; Pathogens; Peptides; Phosphopyruvate hydratase; Phylogeny; Prevention; Protein families; Proteins; Research and Analysis Methods; Secretome; Secretome - metabolism; Species; Species Specificity; Specificity; Therapeutic targets; Transmission; Transthyretin; Tropical diseases; Vaccines; Workflow; Worms
• ISSN: 1935-2735; 1935-2727; 1935-2735
• DOI: 10.1371/journal.pntd.0009828

Parasitic nematodes are highly successful pathogens, inflicting disease on humans, animals and plants. Despite great differences in their life cycles, host preference and transmission modes, these parasites share a common capacity to manipulate their host's immune system. This is at least partly achieved through the release of excretory/secretory proteins, the most well-characterized component of nematode secretomes, that are comprised of functionally diverse molecules. In this work, we analyzed published protein secretomes of parasitic nematodes to identify common patterns as well as species-specific traits. The 20 selected organisms span 4 nematode clades, including plant pathogens, animal parasites, and the free-living species Caenorhabditis elegans. Transthyretin-like proteins were the only component common to all adult secretomes; many other protein classes overlapped across multiple datasets. The glycolytic enzymes aldolase and enolase were present in all parasitic species, but missing from C. elegans. Secretomes from larval stages showed less overlap between species. Although comparison of secretome composition across species and life-cycle stages is challenged by the use of different methods and depths of sequencing among studies, our workflow enabled the identification of conserved protein families and pinpointed elements that may have evolved as to enable parasitism. This strategy, extended to more secretomes, may be exploited to prioritize therapeutic targets in the future.