IGSF11 is required for pericentric heterochromatin dissociation during meiotic diplotene

Meiosis initiation and progression are regulated by both germ cells and gonadal somatic cells. However, little is known about what genes or proteins connecting somatic and germ cells are required for this regulation. Our results show that deficiency for adhesion molecule IGSF11, which is expressed i...

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Bibliographic Details
Published in:PLoS genetics 2021-09, Vol.17 (9), p.e1009778-e1009778
Main Authors: Chen, Bo, Zhu, Gengzhen, Yan, An, He, Jing, Liu, Yang, Li, Lin, Yang, Xuerui, Dong, Chen, Kee, Kehkooi
Format: Article
Language:English
Subjects:
Animals
Biology and Life Sciences
Cell adhesion & migration
Cell Adhesion Molecules - genetics
Cell Adhesion Molecules - physiology
Chromatin
Chromosomes
Engineering and Technology
Female
Genes, Reporter
Genetic aspects
Germ cells
Heterochromatin
Heterochromatin - metabolism
Humans
Immunoglobulins
Immunoglobulins - genetics
Immunoglobulins - physiology
Infertility
Localization
Male
Medicine and Health Sciences
Meiosis
Meiotic Prophase I
Mice
Ovaries
Pachytene
Physiological aspects
Proteins
Sertoli cells
Sertoli Cells - metabolism
Somatic cells
Spermatocytes
Spermatocytes - metabolism
Transgenic animals
Transplantation
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Summary:Meiosis initiation and progression are regulated by both germ cells and gonadal somatic cells. However, little is known about what genes or proteins connecting somatic and germ cells are required for this regulation. Our results show that deficiency for adhesion molecule IGSF11, which is expressed in both Sertoli cells and germ cells, leads to male infertility in mice. Combining a new meiotic fluorescent reporter system with testicular cell transplantation, we demonstrated that IGSF11 is required in both somatic cells and spermatogenic cells for primary spermatocyte development. In the absence of IGSF11, spermatocytes proceed through pachytene, but the pericentric heterochromatin of nonhomologous chromosomes remains inappropriately clustered from late pachytene onward, resulting in undissolved interchromosomal interactions. Hi-C analysis reveals elevated levels of interchromosomal interactions occurring mostly at the chromosome ends. Collectively, our data elucidates that IGSF11 in somatic cells and germ cells is required for pericentric heterochromatin dissociation during diplotene in mouse primary spermatocytes.
ISSN:1553-7404
1553-7390
1553-7404
DOI:10.1371/journal.pgen.1009778