The chromatin remodeler Chd1 supports MRX and Exo1 functions in resection of DNA double-strand breaks

Repair of DNA double-strand breaks (DSBs) by homologous recombination (HR) requires that the 5'-terminated DNA strands are resected to generate single-stranded DNA overhangs. This process is initiated by a short-range resection catalyzed by the MRX (Mre11-Rad50-Xrs2) complex, which is followed...

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Published in:PLoS genetics 2021-09, Vol.17 (9), p.e1009807-e1009807
Main Authors: Gnugnoli, Marco, Casari, Erika, Longhese, Maria Pia
Format: Article
Language:English
Subjects:
Adenosine triphosphatase
Biology and life sciences
Chromatin
Chromatin remodeling
Chromosomes
DNA binding proteins
DNA Breaks, Double-Stranded
DNA damage
DNA repair
DNA-Binding Proteins - physiology
Efficiency
Evictions
Exodeoxyribonucleases - metabolism
Experiments
Genes, Fungal
Genetic research
Histones
Histones - isolation & purification
Homologous recombination
Medicine and Health Sciences
MRE11 protein
Nuclease
Physical sciences
Prostate cancer
Proteins
RNA polymerase
Saccharomyces cerevisiae - genetics
Saccharomyces cerevisiae Proteins - physiology
Single-stranded DNA
Structure
Yeast
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description Repair of DNA double-strand breaks (DSBs) by homologous recombination (HR) requires that the 5'-terminated DNA strands are resected to generate single-stranded DNA overhangs. This process is initiated by a short-range resection catalyzed by the MRX (Mre11-Rad50-Xrs2) complex, which is followed by a long-range step involving the nucleases Exo1 and Dna2. Here we show that the Saccharomyces cerevisiae ATP-dependent chromatin-remodeling protein Chd1 participates in both short- and long-range resection by promoting MRX and Exo1 association with the DSB ends. Furthermore, Chd1 reduces histone occupancy near the DSB ends and promotes DSB repair by HR. All these functions require Chd1 ATPase activity, supporting a role for Chd1 in the opening of chromatin at the DSB site to facilitate MRX and Exo1 processing activities.
doi_str_mv 10.1371/journal.pgen.1009807
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subjects Adenosine triphosphatase
Biology and life sciences
Chromatin
Chromatin remodeling
Chromosomes
DNA binding proteins
DNA Breaks, Double-Stranded
DNA damage
DNA repair
DNA-Binding Proteins - physiology
Efficiency
Evictions
Exodeoxyribonucleases - metabolism
Experiments
Genes, Fungal
Genetic research
Histones
Histones - isolation & purification
Homologous recombination
Medicine and Health Sciences
MRE11 protein
Nuclease
Physical sciences
Prostate cancer
Proteins
RNA polymerase
Saccharomyces cerevisiae - genetics
Saccharomyces cerevisiae Proteins - physiology
Single-stranded DNA
Structure
Yeast
title The chromatin remodeler Chd1 supports MRX and Exo1 functions in resection of DNA double-strand breaks
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