Loading…
Clinical characteristics and viral load dynamics of COVID-19 in a mildly or moderately symptomatic outpatient sample
Studies of outpatients with mild or moderate COVID-19 are uncommon. We studied: 1) association of symptoms with reverse transcriptase polymerase chain reaction (RT-PCR) test results; and 2) association of initial RT-PCR cycle threshold (Ct) in relation to duration of RT-PCR positivity in outpatients...
Saved in:
| Published in: | PloS one 2021-10, Vol.16 (10), p.e0258970-e0258970 |
|---|---|
| Main Authors: | , , , , , , , , |
| Format: | Article |
| Language: | English |
| Subjects: | |
| Citations: | Items that this one cites Items that cite this one |
| Online Access: | Get full text |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| cited_by | cdi_FETCH-LOGICAL-c6207-b1c47aaf77114bada926cb84b51d8dfa1255198f05aaf05dbe33bb22b59144893 |
|---|---|
| cites | cdi_FETCH-LOGICAL-c6207-b1c47aaf77114bada926cb84b51d8dfa1255198f05aaf05dbe33bb22b59144893 |
| container_end_page | e0258970 |
| container_issue | 10 |
| container_start_page | e0258970 |
| container_title | PloS one |
| container_volume | 16 |
| creator | Caplan, Amanda Bates, Kelly W Brioni, Carla Santos, Aileen Sabatini, Linda M Kaul, Karen L Carnethon, Mercedes R Khandekar, Janardan D Greenland, Philip |
| description | Studies of outpatients with mild or moderate COVID-19 are uncommon. We studied: 1) association of symptoms with reverse transcriptase polymerase chain reaction (RT-PCR) test results; and 2) association of initial RT-PCR cycle threshold (Ct) in relation to duration of RT-PCR positivity in outpatients with mild or moderate COVID-19. This was a cohort study of outpatients with confirmed COVID-19 and at least one symptom. Participants had repeat nasopharyngeal swabs and symptom checklists every 3-5 days until two consecutive RT-PCR tests were negative. RT-PCR tests were used to assess viral load. Antibody tests for COVID-19 were performed at 2 weeks, 4 weeks, and 8 weeks after symptom onset. Twenty-five patients (nine females) were enrolled, ranging in age from 19-58 (median age 28 years). All patients reported at least one symptom, with a median of six symptoms per patient. Symptoms persisted for 6-67 days (median duration 18 days). In all 25 patients, blood samples collected a median of 13 days after symptom onset were positive for SARS-CoV-2 antibodies in 15 (60%). After a median of 28 days following symptom onset, 23/23 patients with available samples tested positive for antibodies. The longest duration of positive RT-PCR test was 49 days from first positive PCR test (Mean = 27.4, SD = 12.5, Median = 24). Initial Ct was significantly associated with longer duration ([beta] = -1.3, SE = 0.3, p |
| doi_str_mv | 10.1371/journal.pone.0258970 |
| format | article |
| fullrecord | <record><control><sourceid>gale_plos_</sourceid><recordid>TN_cdi_plos_journals_2584299895</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A679769368</galeid><doaj_id>oai_doaj_org_article_2115abcf2f5240b3980b069fe2b627eb</doaj_id><sourcerecordid>A679769368</sourcerecordid><originalsourceid>FETCH-LOGICAL-c6207-b1c47aaf77114bada926cb84b51d8dfa1255198f05aaf05dbe33bb22b59144893</originalsourceid><addsrcrecordid>eNqNk1uLEzEUxwdR3HX1GwgGBNGH1lzmlhdhqbfCQsHLvoaTy7RZMpOaZBb77U3tKDuyDzIPSU5--Z-cf-YUxXOCl4Q15O2NH8MAbrn3g1liWrW8wQ-Kc8IZXdQUs4d35mfFkxhvMK5YW9ePizNW1g1rSX1epJWzg1XgkNpBAJVMsDFZFREMGt3akHecB430YYD-GPcdWm2u1-8XhCM7IEC9ddodkA-o99oESCav4qHfJ99DlkJ-TPs8MUNCEfq9M0-LRx24aJ5N40Xx_eOHb6vPi6vNp_Xq8mqh8qWbhSSqbAC6piGklKCB01rJtpQV0a3ugNCqIrztcJUhXGlpGJOSUllxUpYtZxfFi5Pu3vkoJsOiyF6VlPOWV5lYnwjt4Ubsg-0hHIQHK34HfNgKCLkGZwQlpAKpOtpVtMSS8RZLXPPOUFnTxsis9W7KNsreaJXrze7NROc7g92Jrb8VbcUwK9ss8HoSCP7HaGISvY3KOAeD8ePp3iVrKKMZffkPen91E7WFXIAdOp_zqqOouKwb3tSc1ce0y3uo_GmTHzz_XZ3N8dmBN7MDmUnmZ9rCGKNYf_3y_-zmes6-usPuDLi0i96NyfohzsHyBKrgYwym-2syweLYHH_cEMfmEFNzsF8n5wCx</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2584299895</pqid></control><display><type>article</type><title>Clinical characteristics and viral load dynamics of COVID-19 in a mildly or moderately symptomatic outpatient sample</title><source>Open Access: PubMed Central</source><source>Publicly Available Content Database</source><source>Coronavirus Research Database</source><creator>Caplan, Amanda ; Bates, Kelly W ; Brioni, Carla ; Santos, Aileen ; Sabatini, Linda M ; Kaul, Karen L ; Carnethon, Mercedes R ; Khandekar, Janardan D ; Greenland, Philip</creator><contributor>Roques, Pierre</contributor><creatorcontrib>Caplan, Amanda ; Bates, Kelly W ; Brioni, Carla ; Santos, Aileen ; Sabatini, Linda M ; Kaul, Karen L ; Carnethon, Mercedes R ; Khandekar, Janardan D ; Greenland, Philip ; Roques, Pierre</creatorcontrib><description>Studies of outpatients with mild or moderate COVID-19 are uncommon. We studied: 1) association of symptoms with reverse transcriptase polymerase chain reaction (RT-PCR) test results; and 2) association of initial RT-PCR cycle threshold (Ct) in relation to duration of RT-PCR positivity in outpatients with mild or moderate COVID-19. This was a cohort study of outpatients with confirmed COVID-19 and at least one symptom. Participants had repeat nasopharyngeal swabs and symptom checklists every 3-5 days until two consecutive RT-PCR tests were negative. RT-PCR tests were used to assess viral load. Antibody tests for COVID-19 were performed at 2 weeks, 4 weeks, and 8 weeks after symptom onset. Twenty-five patients (nine females) were enrolled, ranging in age from 19-58 (median age 28 years). All patients reported at least one symptom, with a median of six symptoms per patient. Symptoms persisted for 6-67 days (median duration 18 days). In all 25 patients, blood samples collected a median of 13 days after symptom onset were positive for SARS-CoV-2 antibodies in 15 (60%). After a median of 28 days following symptom onset, 23/23 patients with available samples tested positive for antibodies. The longest duration of positive RT-PCR test was 49 days from first positive PCR test (Mean = 27.4, SD = 12.5, Median = 24). Initial Ct was significantly associated with longer duration ([beta] = -1.3, SE = 0.3, p<0.01 per 1 cycle higher) of RT-PCR positivity. In mildly or moderately ill COVID-19 outpatients, RT-PCT tests remained positive for as long as 49 days and test positivity and symptom duration correlated with initial viral load.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0258970</identifier><identifier>PMID: 34673816</identifier><language>eng</language><publisher>San Francisco: Public Library of Science</publisher><subject>Antibodies ; Care and treatment ; Check lists ; Coronaviruses ; COVID-19 ; Disease control ; DNA polymerases ; Headaches ; Health care ; Hospitalization ; Illnesses ; Infections ; Measurement ; Medical laboratories ; Outpatients ; Patients ; Polymerase chain reaction ; Preventive medicine ; RNA-directed DNA polymerase ; Severe acute respiratory syndrome coronavirus 2 ; Testing ; Viral antibodies ; Viral diseases</subject><ispartof>PloS one, 2021-10, Vol.16 (10), p.e0258970-e0258970</ispartof><rights>COPYRIGHT 2021 Public Library of Science</rights><rights>2021 Caplan et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2021 Caplan et al 2021 Caplan et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c6207-b1c47aaf77114bada926cb84b51d8dfa1255198f05aaf05dbe33bb22b59144893</citedby><cites>FETCH-LOGICAL-c6207-b1c47aaf77114bada926cb84b51d8dfa1255198f05aaf05dbe33bb22b59144893</cites><orcidid>0000-0002-6327-2439</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.proquest.com/docview/2584299895?pq-origsite=primo$$EPDF$$P50$$Gproquest$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.proquest.com/docview/2584299895?pq-origsite=primo$$EHTML$$P50$$Gproquest$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,25753,27924,27925,37012,37013,38516,43895,44590,53791,53793,74412,75126</link.rule.ids></links><search><contributor>Roques, Pierre</contributor><creatorcontrib>Caplan, Amanda</creatorcontrib><creatorcontrib>Bates, Kelly W</creatorcontrib><creatorcontrib>Brioni, Carla</creatorcontrib><creatorcontrib>Santos, Aileen</creatorcontrib><creatorcontrib>Sabatini, Linda M</creatorcontrib><creatorcontrib>Kaul, Karen L</creatorcontrib><creatorcontrib>Carnethon, Mercedes R</creatorcontrib><creatorcontrib>Khandekar, Janardan D</creatorcontrib><creatorcontrib>Greenland, Philip</creatorcontrib><title>Clinical characteristics and viral load dynamics of COVID-19 in a mildly or moderately symptomatic outpatient sample</title><title>PloS one</title><description>Studies of outpatients with mild or moderate COVID-19 are uncommon. We studied: 1) association of symptoms with reverse transcriptase polymerase chain reaction (RT-PCR) test results; and 2) association of initial RT-PCR cycle threshold (Ct) in relation to duration of RT-PCR positivity in outpatients with mild or moderate COVID-19. This was a cohort study of outpatients with confirmed COVID-19 and at least one symptom. Participants had repeat nasopharyngeal swabs and symptom checklists every 3-5 days until two consecutive RT-PCR tests were negative. RT-PCR tests were used to assess viral load. Antibody tests for COVID-19 were performed at 2 weeks, 4 weeks, and 8 weeks after symptom onset. Twenty-five patients (nine females) were enrolled, ranging in age from 19-58 (median age 28 years). All patients reported at least one symptom, with a median of six symptoms per patient. Symptoms persisted for 6-67 days (median duration 18 days). In all 25 patients, blood samples collected a median of 13 days after symptom onset were positive for SARS-CoV-2 antibodies in 15 (60%). After a median of 28 days following symptom onset, 23/23 patients with available samples tested positive for antibodies. The longest duration of positive RT-PCR test was 49 days from first positive PCR test (Mean = 27.4, SD = 12.5, Median = 24). Initial Ct was significantly associated with longer duration ([beta] = -1.3, SE = 0.3, p<0.01 per 1 cycle higher) of RT-PCR positivity. In mildly or moderately ill COVID-19 outpatients, RT-PCT tests remained positive for as long as 49 days and test positivity and symptom duration correlated with initial viral load.</description><subject>Antibodies</subject><subject>Care and treatment</subject><subject>Check lists</subject><subject>Coronaviruses</subject><subject>COVID-19</subject><subject>Disease control</subject><subject>DNA polymerases</subject><subject>Headaches</subject><subject>Health care</subject><subject>Hospitalization</subject><subject>Illnesses</subject><subject>Infections</subject><subject>Measurement</subject><subject>Medical laboratories</subject><subject>Outpatients</subject><subject>Patients</subject><subject>Polymerase chain reaction</subject><subject>Preventive medicine</subject><subject>RNA-directed DNA polymerase</subject><subject>Severe acute respiratory syndrome coronavirus 2</subject><subject>Testing</subject><subject>Viral antibodies</subject><subject>Viral diseases</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>COVID</sourceid><sourceid>PIMPY</sourceid><sourceid>DOA</sourceid><recordid>eNqNk1uLEzEUxwdR3HX1GwgGBNGH1lzmlhdhqbfCQsHLvoaTy7RZMpOaZBb77U3tKDuyDzIPSU5--Z-cf-YUxXOCl4Q15O2NH8MAbrn3g1liWrW8wQ-Kc8IZXdQUs4d35mfFkxhvMK5YW9ePizNW1g1rSX1epJWzg1XgkNpBAJVMsDFZFREMGt3akHecB430YYD-GPcdWm2u1-8XhCM7IEC9ddodkA-o99oESCav4qHfJ99DlkJ-TPs8MUNCEfq9M0-LRx24aJ5N40Xx_eOHb6vPi6vNp_Xq8mqh8qWbhSSqbAC6piGklKCB01rJtpQV0a3ugNCqIrztcJUhXGlpGJOSUllxUpYtZxfFi5Pu3vkoJsOiyF6VlPOWV5lYnwjt4Ubsg-0hHIQHK34HfNgKCLkGZwQlpAKpOtpVtMSS8RZLXPPOUFnTxsis9W7KNsreaJXrze7NROc7g92Jrb8VbcUwK9ss8HoSCP7HaGISvY3KOAeD8ePp3iVrKKMZffkPen91E7WFXIAdOp_zqqOouKwb3tSc1ce0y3uo_GmTHzz_XZ3N8dmBN7MDmUnmZ9rCGKNYf_3y_-zmes6-usPuDLi0i96NyfohzsHyBKrgYwym-2syweLYHH_cEMfmEFNzsF8n5wCx</recordid><startdate>20211021</startdate><enddate>20211021</enddate><creator>Caplan, Amanda</creator><creator>Bates, Kelly W</creator><creator>Brioni, Carla</creator><creator>Santos, Aileen</creator><creator>Sabatini, Linda M</creator><creator>Kaul, Karen L</creator><creator>Carnethon, Mercedes R</creator><creator>Khandekar, Janardan D</creator><creator>Greenland, Philip</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>COVID</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope><orcidid>https://orcid.org/0000-0002-6327-2439</orcidid></search><sort><creationdate>20211021</creationdate><title>Clinical characteristics and viral load dynamics of COVID-19 in a mildly or moderately symptomatic outpatient sample</title><author>Caplan, Amanda ; Bates, Kelly W ; Brioni, Carla ; Santos, Aileen ; Sabatini, Linda M ; Kaul, Karen L ; Carnethon, Mercedes R ; Khandekar, Janardan D ; Greenland, Philip</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c6207-b1c47aaf77114bada926cb84b51d8dfa1255198f05aaf05dbe33bb22b59144893</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Antibodies</topic><topic>Care and treatment</topic><topic>Check lists</topic><topic>Coronaviruses</topic><topic>COVID-19</topic><topic>Disease control</topic><topic>DNA polymerases</topic><topic>Headaches</topic><topic>Health care</topic><topic>Hospitalization</topic><topic>Illnesses</topic><topic>Infections</topic><topic>Measurement</topic><topic>Medical laboratories</topic><topic>Outpatients</topic><topic>Patients</topic><topic>Polymerase chain reaction</topic><topic>Preventive medicine</topic><topic>RNA-directed DNA polymerase</topic><topic>Severe acute respiratory syndrome coronavirus 2</topic><topic>Testing</topic><topic>Viral antibodies</topic><topic>Viral diseases</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Caplan, Amanda</creatorcontrib><creatorcontrib>Bates, Kelly W</creatorcontrib><creatorcontrib>Brioni, Carla</creatorcontrib><creatorcontrib>Santos, Aileen</creatorcontrib><creatorcontrib>Sabatini, Linda M</creatorcontrib><creatorcontrib>Kaul, Karen L</creatorcontrib><creatorcontrib>Carnethon, Mercedes R</creatorcontrib><creatorcontrib>Khandekar, Janardan D</creatorcontrib><creatorcontrib>Greenland, Philip</creatorcontrib><collection>CrossRef</collection><collection>Gale in Context : Opposing Viewpoints</collection><collection>Gale In Context: Science</collection><collection>ProQuest Central (Corporate)</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Nursing & Allied Health Database</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Meteorological & Geoastrophysical Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central</collection><collection>Advanced Technologies & Aerospace Database (1962 - current)</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>AUTh Library subscriptions: ProQuest Central</collection><collection>Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>Coronavirus Research Database</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Materials Science Database</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Meteorological & Geoastrophysical Abstracts - Academic</collection><collection>ProQuest Engineering Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>PML(ProQuest Medical Library)</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Engineering Database</collection><collection>Nursing & Allied Health Premium</collection><collection>ProQuest advanced technologies & aerospace journals</collection><collection>ProQuest Advanced Technologies & Aerospace Collection</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environmental Science Database</collection><collection>Materials science collection</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Engineering collection</collection><collection>Environmental Science Collection</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Caplan, Amanda</au><au>Bates, Kelly W</au><au>Brioni, Carla</au><au>Santos, Aileen</au><au>Sabatini, Linda M</au><au>Kaul, Karen L</au><au>Carnethon, Mercedes R</au><au>Khandekar, Janardan D</au><au>Greenland, Philip</au><au>Roques, Pierre</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Clinical characteristics and viral load dynamics of COVID-19 in a mildly or moderately symptomatic outpatient sample</atitle><jtitle>PloS one</jtitle><date>2021-10-21</date><risdate>2021</risdate><volume>16</volume><issue>10</issue><spage>e0258970</spage><epage>e0258970</epage><pages>e0258970-e0258970</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Studies of outpatients with mild or moderate COVID-19 are uncommon. We studied: 1) association of symptoms with reverse transcriptase polymerase chain reaction (RT-PCR) test results; and 2) association of initial RT-PCR cycle threshold (Ct) in relation to duration of RT-PCR positivity in outpatients with mild or moderate COVID-19. This was a cohort study of outpatients with confirmed COVID-19 and at least one symptom. Participants had repeat nasopharyngeal swabs and symptom checklists every 3-5 days until two consecutive RT-PCR tests were negative. RT-PCR tests were used to assess viral load. Antibody tests for COVID-19 were performed at 2 weeks, 4 weeks, and 8 weeks after symptom onset. Twenty-five patients (nine females) were enrolled, ranging in age from 19-58 (median age 28 years). All patients reported at least one symptom, with a median of six symptoms per patient. Symptoms persisted for 6-67 days (median duration 18 days). In all 25 patients, blood samples collected a median of 13 days after symptom onset were positive for SARS-CoV-2 antibodies in 15 (60%). After a median of 28 days following symptom onset, 23/23 patients with available samples tested positive for antibodies. The longest duration of positive RT-PCR test was 49 days from first positive PCR test (Mean = 27.4, SD = 12.5, Median = 24). Initial Ct was significantly associated with longer duration ([beta] = -1.3, SE = 0.3, p<0.01 per 1 cycle higher) of RT-PCR positivity. In mildly or moderately ill COVID-19 outpatients, RT-PCT tests remained positive for as long as 49 days and test positivity and symptom duration correlated with initial viral load.</abstract><cop>San Francisco</cop><pub>Public Library of Science</pub><pmid>34673816</pmid><doi>10.1371/journal.pone.0258970</doi><tpages>e0258970</tpages><orcidid>https://orcid.org/0000-0002-6327-2439</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2021-10, Vol.16 (10), p.e0258970-e0258970 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_2584299895 |
| source | Open Access: PubMed Central; Publicly Available Content Database; Coronavirus Research Database |
| subjects | Antibodies Care and treatment Check lists Coronaviruses COVID-19 Disease control DNA polymerases Headaches Health care Hospitalization Illnesses Infections Measurement Medical laboratories Outpatients Patients Polymerase chain reaction Preventive medicine RNA-directed DNA polymerase Severe acute respiratory syndrome coronavirus 2 Testing Viral antibodies Viral diseases |
| title | Clinical characteristics and viral load dynamics of COVID-19 in a mildly or moderately symptomatic outpatient sample |
| url | http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-20T22%3A11%3A20IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Clinical%20characteristics%20and%20viral%20load%20dynamics%20of%20COVID-19%20in%20a%20mildly%20or%20moderately%20symptomatic%20outpatient%20sample&rft.jtitle=PloS%20one&rft.au=Caplan,%20Amanda&rft.date=2021-10-21&rft.volume=16&rft.issue=10&rft.spage=e0258970&rft.epage=e0258970&rft.pages=e0258970-e0258970&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0258970&rft_dat=%3Cgale_plos_%3EA679769368%3C/gale_plos_%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c6207-b1c47aaf77114bada926cb84b51d8dfa1255198f05aaf05dbe33bb22b59144893%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=2584299895&rft_id=info:pmid/34673816&rft_galeid=A679769368&rfr_iscdi=true |