Cytotoxin antibody-based colourimetric sensor for field-level differential detection of elapid among big four snake venom

Development of a rapid, on-site detection tool for snakebite is highly sought after, owing to its clinically and forensically relevant medicolegal significance. Polyvalent antivenom therapy in the management of such envenomation cases is finite due to its poor venom neutralization capabilities as we...

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Bibliographic Details
Published in:PLoS neglected tropical diseases 2021-10, Vol.15 (10), p.e0009841
Main Authors: Kaul, Sherin, Sai Keerthana, L, Kumar, Pankaj, Birader, Komal, Tammineni, Yathirajarao, Rawat, Deepali, Suman, Pankaj
Format: Article
Language:English
Subjects:
Animals
Antibodies, Monoclonal - analysis
Antibodies, Monoclonal - immunology
Antivenom
Biology and Life Sciences
Bites and stings
Bungarus - genetics
Bungarus - physiology
Chemical detectors
Cloning
Colorimetry
Colorimetry - methods
Cytotoxins - analysis
Cytotoxins - genetics
Cytotoxins - immunology
Detection
Diagnosis
E coli
Elapid Venoms - analysis
Elapid Venoms - genetics
Elapid Venoms - immunology
Elapidae - immunology
Elapidae - physiology
Identification
Immunoassay - methods
Immunoglobulin G
Immunology
Immunoreactivity
Immunotoxins - analysis
Immunotoxins - genetics
Immunotoxins - immunology
Industrial production
Medicine and Health Sciences
Methods
Mitochondrial DNA
Molecular diagnostic techniques
Monoclonal antibodies
Naja naja
Naja naja - immunology
Naja naja - physiology
Neutralization
Polyclonal antibodies
Proteins
Quantitation
Recombinants
Research and Analysis Methods
Samples
Serum
Snake bites
Snake Venoms - analysis
Snake Venoms - immunology
Snakes
Therapy
Tropical diseases
Venom
Venomous snakes
Viperidae - immunology
Viperidae - physiology
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Summary:Development of a rapid, on-site detection tool for snakebite is highly sought after, owing to its clinically and forensically relevant medicolegal significance. Polyvalent antivenom therapy in the management of such envenomation cases is finite due to its poor venom neutralization capabilities as well as diagnostic ramifications manifested as untoward immunological reactions. For precise molecular diagnosis of elapid venoms of the big four snakes, we have developed a lateral flow kit using a monoclonal antibody (AB1; IgG1 - κ chain; Kd: 31 nM) generated against recombinant cytotoxin-7 (rCTX-7; 7.7 kDa) protein of the elapid venom. The monoclonal antibody specifically detected the venoms of Naja naja (p < 0.0001) and Bungarus caeruleus (p
ISSN:1935-2735
1935-2727
1935-2735
DOI:10.1371/journal.pntd.0009841