Diversity and distribution of the tick-borne relapsing fever spirochete Borrelia turicatae

• Published in: PLoS neglected tropical diseases 2021-11, Vol.15 (11), p.e0009868
• Main Authors: Krishnavajhala, Aparna, Armstrong, Brittany A, Kneubehl, Alexander R, Gunter, Sarah M, Piccione, Julie, Kim, Hee J, Ramirez, Rosa, Castro-Arellano, Ivan, Roachell, Walter, Teel, Pete D, Lopez, Job E
• Format: Article
• Language: English
• Subjects: Animals; Arachnids; Bacterial Proteins - genetics; Bacterial Proteins - metabolism; Biodiversity; Biology and Life Sciences; Border patrol; Borrelia; Borrelia - classification; Borrelia - genetics; Borrelia - isolation & purification; Borrelia - metabolism; Caves; Chromosomes; Computer and Information Sciences; Deoxyribonucleic acid; Diagnosis; Distribution; DNA; DNA topoisomerase; Female; Fever; Flagellin; Genomes; Glycerol; Homeless people; Homelessness; Humans; Jarisch-Herxheimer reaction; Laboratory animals; Loci; Male; Medicine and Health Sciences; Multilocus Sequence Typing; Nucleotide sequence; Parks; Parks & recreation areas; Pathogens; People and places; Phylogenetics; Phylogeny; Plasmids; Plasmids - genetics; Plasmids - metabolism; Population density; Public health; Recreation; Recreation areas; Recreational areas; Relapsing fever; Relapsing Fever - microbiology; Relapsing Fever - transmission; rRNA 16S; Rural areas; Spirochetes; Texas; Tick-borne diseases; Ticks; Ticks - microbiology; Ticks - physiology; Tropical diseases; Tropical environments; Urban areas
• ISSN: 1935-2735; 1935-2727; 1935-2735
• DOI: 10.1371/journal.pntd.0009868

Borrelia turicatae is a causative agent of tick-borne relapsing fever (TBRF) in the subtropics and tropics of the United States and Latin America. Historically, B. turicatae was thought to be maintained in enzootic cycles in rural areas. However, there is growing evidence that suggests the pathogen has established endemic foci in densely populated regions of Texas. With the growth of homelessness in the state and human activity in city parks, it was important to implement field collection efforts to identify areas where B. turicatae and its vector circulate. Between 2017 and 2020 we collected Ornithodoros turicata ticks in suburban and urban areas including public and private parks and recreational spaces. Ticks were fed on naïve mice and spirochetes were isolated from the blood. Multilocus sequence typing (MLST) was performed on eight newly obtained isolates and included previously reported sequences. The four chromosomal loci targeted for MLST were 16S ribosomal RNA (rrs), flagellin B (flaB), DNA gyrase B (gyrB), and the intergenic spacer (IGS). Given the complexity of Borrelia genomes, plasmid diversity was also evaluated. These studies indicate that the IGS locus segregates B. turicatae into four genomic types and plasmid diversity is extensive between isolates. Furthermore, B. turicatae and its vector have established endemic foci in parks and recreational areas in densely populated settings of Texas.