Sterilized human skin graft with a dose of 25 kGy provides a privileged immune and collagen microenvironment in the adhesion of Nude mice wounds

• Published in: PloS one 2022-01, Vol.17 (1), p.e0262532-e0262532
• Main Authors: Tomaz de Miranda, Jurandir, Bringel, Fabiana de Andrade, Pereira Velosa, Ana Paula, Protocevich, Verônica, Fernezlian, Sandra de Morais, Silva, Pedro Leme, Capelozzi, Vera Luiza, Mathor, Monica Beatriz, Teodoro, Walcy Rosolia
• Format: Article
• Language: English
• Subjects: Adhesion; Animal models; Animals; Auroral kilometric radiation; Bioengineering; Biology and Life Sciences; Biomechanics; Cellular Microenvironment - physiology; Collagen; Collagen (type I); Collagen Type I - metabolism; Drug dosages; Energy; Ethics; Female; Fibroblasts; Grafting; Granulation; Health aspects; Hospitals; Humans; Inflammation; Ionizing radiation; Leukocytes (neutrophilic); Lymphocytes B; Macrophages; Male; Medicine and Health Sciences; Methods; Mice; Mice, Nude; Microenvironments; Necrosis; Nuclear physics; Nuclear research; Physiological aspects; Physiology; Plastic surgery; Radiation; Re-Epithelialization - physiology; Research and Analysis Methods; Rheumatology; Skin; Skin & tissue grafts; Skin - metabolism; Skin - physiopathology; Skin grafts; Skin Transplantation - methods; Skin, Artificial; Skin-grafting; Thickness; Tissue Adhesions - metabolism; Tissue Adhesions - physiopathology; Titanium alloys; Transplantation; Vascularization; Wound healing; Wound Healing - physiology
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0262532

This study aimed to report the effects of different doses of ionizing radiation on inflammatory and repair stage of human skin graft adherence in Nude mice wounds. Animals were divided into transplanted with irradiated human skin grafts (IHSG) at 25 and 50 kGy (IHSG 25 kGy; IHSG 50 kGy) and non-IHSG and euthanized on the 3rd, 7th and 21st days after the surgery, by gross and microscopic changes, immunostaining for human type I collagen (Col I) and mouse Col I and Col III and inflammatory cells. We found an effectiveness of human split-thickness graft adherence in mice transplanted with IHSG 25 kGy, as well decrease in dermo-epidermal necrosis and neutrophils, lower loss of skin thickness, epithelization and neo-vascularization. Day 21 post-transplantation with IHSG 25 kGy was observed a well-preserved human skin in the border of the graft, a prominent granulation tissue in an organization by proliferated fibroblasts, Col III deposition and increased B-cells and macrophages. A complete adherence of human skin graft occurred with IHSG 25 kGy. We suggest that the ionizing radiation at 25 kGy mediates inflammation and the repair stage of human skin graft adherence in murine model, thus emerging as a potential tool in healing cutaneous wounds.