Bifidobacterium lactis BL-99 modulates intestinal inflammation and functions in zebrafish models

• Published in: PloS one 2022-02, Vol.17 (2), p.e0262942
• Main Authors: Chen, Meng, Liu, Chinfeng, Dai, Mingzhu, Wang, Qinwen, Li, Chunqi, Hung, Weilian
• Format: Article
• Language: English
• Subjects: Accreditation; Acid resistance; Acids; Alum Compounds - toxicity; Aluminum; Aluminum sulfate; Analysis; Animals; Bacteria; Bifidobacterium; Bifidobacterium - physiology; Bifidobacterium lactis; Biology and Life Sciences; Biotechnology; Constipation; Constipation - chemically induced; Constipation - pathology; Constipation - therapy; Danio rerio; Diagnosis; Discriminant Analysis; Disease Models, Animal; Down-Regulation - drug effects; Drug resistance; Gastric juice; Gastrointestinal Motility - drug effects; Gene expression; Genetic aspects; Glucose - pharmacology; Health aspects; Histopathology; IL-1β; Inflammation; Inflammation - chemically induced; Inflammation - pathology; Inflammation - therapy; Inflammatory bowel disease; Interleukin 10; Interleukin 12; Interleukin-10 - genetics; Interleukin-10 - metabolism; Interleukin-1beta - genetics; Interleukin-1beta - metabolism; Intestinal motility; Intestine; Intestines - metabolism; Intestines - microbiology; Intestines - pathology; Laboratory animals; Larva - drug effects; Larva - metabolism; Mammals; Medicine and Health Sciences; Metabolic disorders; Metabolites; Microbiota; Microorganisms; Modelling; Motility; Probiotics; Probiotics - pharmacology; Probiotics - therapeutic use; Research and Analysis Methods; Sulfates; Tryptase; Up-Regulation - drug effects; Zebra fish; Zebrafish; Zebrafish - growth & development
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0262942

This study was designed to explore the therapeutics and the mechanisms of a patented and marked gastric acid and intestine juice-resistant probiotics Bifidobacterium lactis BL-99 (B. lactis BL-99) on the intestinal inflammation and functions in the zebrafish models. After feeding for 6 hours, B. lactis BL-99 was fully retained in the larval zebrafish intestinal tract and stayed for over 24 hours. B. lactis BL-99 promoted the intestinal motility and effectively alleviated aluminum sulfate-induced larval zebrafish constipation (p < 0.01). Irregular high glucose diet induced adult zebrafish intestinal functional and metabolic disorders. After fed with B. lactis BL-99, IL-1β gene expression was significantly down-regulated, and IL-10 and IL-12 gene levels were markedly up-regulated in this model (p < 0.05). The intestinal lipase activity was elevated in the adult zebrafish intestinal functional disorder model after B. lactis BL-99 treatment (p < 0.05), but tryptase content had no statistical changes (p > 0.05). B. lactis BL-99 improved the histopathology of the adult zebrafish intestinal inflammation, increased the goblet cell numbers, and up-and-down metabolites were markedly recovered after treatment of B. lactis BL-99 (p < 0.05). These results suggest that B. lactis BL-99 could relieve intestinal inflammation and promote intestinal functions, at least in part, through modulating intestinal and microbial metabolism to maintain intestinal health.