Characterisation of breast cancer molecular signature and treatment assessment with vibrational spectroscopy and chemometric approach

Triple negative breast cancer (TNBC) is regarded as the most aggressive breast cancer subtype with poor overall survival and lack of targeted therapies, resulting in many patients with recurrent. The insight into the detailed biochemical composition of TNBC would help develop dedicated treatments. T...

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Bibliographic Details
Published in:PloS one 2022-03, Vol.17 (3), p.e0264347-e0264347
Main Authors: Kołodziej, Magdalena, Kaznowska, Ewa, Paszek, Sylwia, Cebulski, Józef, Barnaś, Edyta, Cholewa, Marian, Vongsvivut, Jitraporn, Zawlik, Izabela
Format: Article
Language:English
Subjects:
Algorithms
Amides - therapeutic use
Analysis
Biochemical composition
Breast - metabolism
Breast cancer
Cancer
Cancer therapies
Chemometrics
Chemotherapy
Clinical medicine
Cluster analysis
Deoxyribonucleic acid
DNA
Fourier transforms
Gene amplification
Glycogen
Glycogens
Health aspects
Humans
Infrared spectroscopy
Innovations
Measurement
Medical research
Medical schools
Medicine and Health Sciences
Nucleic acids
Patient outcomes
Patients
Physical Sciences
Polysaccharides
Research and Analysis Methods
Saccharides
Spectroscopic analysis
Spectroscopy, Fourier Transform Infrared - methods
Spectrum analysis
Triple Negative Breast Neoplasms - genetics
Vibrational spectra
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Summary:Triple negative breast cancer (TNBC) is regarded as the most aggressive breast cancer subtype with poor overall survival and lack of targeted therapies, resulting in many patients with recurrent. The insight into the detailed biochemical composition of TNBC would help develop dedicated treatments. Thus, in this study Fourier Transform Infrared microspectroscopy combined with chemometrics and absorbance ratios investigation was employed to compare healthy controls with TNBC tissue before and after chemotherapy within the same patient. The primary spectral differences between control and cancer tissues were found in proteins, polysaccharides, and nucleic acids. Amide I/Amide II ratio decrease before and increase after chemotherapy, whereas DNA, RNA, and glycogen contents increase before and decrease after the treatment. The chemometric results revealed discriminatory features reflecting a clinical response scheme and proved the chemotherapy efficacy assessment with infrared spectroscopy is possible.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0264347