Investigation of liposomal self-adjuvanting peptide epitopes derived from conserved blood-stage Plasmodium antigens

Malaria is a vector born parasitic disease causing millions of deaths every year. Despite the high mortality rate, an effective vaccine against this mosquito-borne infectious disease is yet to be developed. Up to date, RTS,S/AS01 is the only vaccine available for malaria prevention; however, its eff...

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Bibliographic Details
Published in:PloS one 2022-03, Vol.17 (3), p.e0264961
Main Authors: Islam, Md Tanjir, Ho, Mei-Fong, Nahar, Ummey J, Shalash, Ahmed O, Koirala, Prashamsa, Hussein, Waleed M, Stanisic, Danielle I, Good, Michael F, Skwarczynski, Mariusz, Toth, Istvan
Format: Article
Language:English
Subjects:
Adjuvants, Immunologic
Amino acids
Animals
Antibodies
Antibodies, Protozoan
Antigens
Antigens, Protozoan
Antigens, Surface
Biology and Life Sciences
Chemistry
Epitopes
Erythrocytes
Evaluation
Health aspects
Immunization
Infectious diseases
Liposomes
Malaria
Malaria - prevention & control
Malaria vaccine
Medicine and Health Sciences
Merozoite Surface Protein 1
Mice
Molecular weight
Parasites
Parasitic diseases
Peptides
Plasmodium
Plasmodium falciparum
Polyethylene glycol
Polyleucine
Prevention
Proteins
Protozoan Proteins
Public health
Research and Analysis Methods
Solvents
Vaccine development
Vaccines
Vaccines, Subunit
Vector-borne diseases
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Summary:Malaria is a vector born parasitic disease causing millions of deaths every year. Despite the high mortality rate, an effective vaccine against this mosquito-borne infectious disease is yet to be developed. Up to date, RTS,S/AS01 is the only vaccine available for malaria prevention; however, its efficacy is low. Among a variety of malaria antigens, merozoite surface protein-1(MSP-1) and ring-infected erythrocyte surface antigen (RESA) have been proposed as promising candidates for malaria vaccine development. We developed peptide-based Plasmodium falciparum vaccine candidates that incorporated three previously reported conserved epitopes from MSP-1 and RESA into highly effective liposomal polyleucine delivery system. Indeed, MSP-1 and RESA-derived epitopes conjugated to polyleucine and formulated into liposomes induced higher epitope specific antibody titres. However, immunized mice failed to demonstrate protection in a rodent malaria challenge study with Plasmodium yoelii. In addition, we found that the three reported P. falciparum epitopes did not to share conformational properties and high sequence similarity with P. yoelii MSP-1 and RESA proteins, despite the epitopes were reported to protect mice against P. yoelii challenge.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0264961