Identification of a coproporphyrinogen-III oxidase gene and its correlation with nacre color in Hyriopsis cumingii

Pearl color is an important factor influencing pearl value, and is affected by the nacre color of the shell in Hyriopsis cumingii. Coproporphyrinogen-III oxidase (CPOX) is a key enzyme in porphyrin synthesis, and porphyrins are involved in color formation in different organisms, including in the nac...

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Published in:PloS one 2022-03, Vol.17 (3), p.e0265318-e0265318
Main Authors: Chen, Xiajun, He, Jixiang, Zhang, Mengying, Bai, Zhiyi, Li, Jiale
Format: Article
Language:English
Subjects:
Agriculture
Amino acid sequence
Amino acids
Analysis
Animals
Aquaculture
Biology and Life Sciences
Bivalvia - genetics
Bivalvia - metabolism
Calcium carbonate
Color
Coproporphyrinogen Oxidase - metabolism
Coproporphyrinogens - metabolism
Earth Sciences
Ecology and Environmental Sciences
Engineering research
Enzymes
Epithelial cells
Epithelium
Fisheries
Genes
Genetic aspects
Hybridization
Hyriopsis cumingii
Identification and classification
Laboratories
Mantle
Medicine and Health Sciences
Mollusks
mRNA
Mussels
Nacre
Nacre - metabolism
Oxidase
Oxidases
Oxidoreductases - metabolism
Pearls
Physical Sciences
Porphyrins
Research and Analysis Methods
RNA-mediated interference
Science education
Synthesis
Unionidae - genetics
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Summary:Pearl color is an important factor influencing pearl value, and is affected by the nacre color of the shell in Hyriopsis cumingii. Coproporphyrinogen-III oxidase (CPOX) is a key enzyme in porphyrin synthesis, and porphyrins are involved in color formation in different organisms, including in the nacre color of mussels. In this study, a CPOX gene (HcCPOX) was identified from H. cumingii, and its amino acid sequence was found to contain a coprogen-oxidase domain. HcCPOX mRNA was expressed widely in the tissues of white and purple mussels, and the highest expression was found in the gill, followed by the fringe mantle. The expression of HcCPOX in all tissues of purple mussels (except in the middle mantle) was higher than that of white mussels. Strong hybridization signals for HcCPOX were observed in the dorsal epithelial cells of the outer fold of the mantle. The activity of CPOX in the gill, fringe mantle, and foot of purple mussels was significantly higher than that in white mussels. Moreover, the expression of HcCPOX and CPOX activity were decreased in RNA interference experiments. The findings indicate that HcCPOX might contributes to nacre color formation in H. cumingii by being involved in porphyrin synthesis.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0265318