Stem cell sheet interpositioned between the tendon and bone would be better for healing than stem cell sheet overlaid above the tendon-to-bone junction in rotator cuff repair of rats

• Published in: PloS one 2022-03, Vol.17 (3), p.e0266030
• Main Authors: Choi, Jae Hee, Shim, In Kyong, Shin, Myung Jin, Lee, Yu Na, Koh, Kyoung Hwan
• Format: Article
• Language: English
• Subjects: Animal models; Animals; Antibodies; Biology and Life Sciences; Biomechanical Phenomena; Biomechanics; Bone growth; Bone healing; Collagen (type III); Complications and side effects; Disease Models, Animal; Flow cytometry; Fluorescence; Gene expression; Green fluorescent protein; Healing; Histology; Humerus; In vivo methods and tests; Life sciences; Medical schools; Medicine; Medicine and Health Sciences; Mesenchymal stem cells; Methods; Patient outcomes; Polymerase chain reaction; Rats; Regeneration; Rotator cuff; Rotator Cuff - surgery; Rotator Cuff Injuries - pathology; Rotator Cuff Injuries - surgery; Rotator cuff repair; Stem cell transplantation; Stem Cells; Tendons - pathology; Transplantation; Transplants & implants; Wound Healing
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0266030

Although stem cells might enhance natural enthesis healing in surgical rotator cuff repair, not much attention has been given to the delivery and location of delivering stem cells. The purpose of this study to know where to locate those stem cells during repair. Animal model of chronic rotator cuff tear was created in 24 rats. Adipose-derived stem cells were engineered as a sheet and transplanted 1) between a torn tendon and humerus (interposition group) or 2) over a repaired tendon-to-bone junction (overlay group) at the time of surgical repair. Tracking of stem cells with overexpression of green fluorescent protein (GFP) were carried out at the time of sacrifice in additional 4 shoulders in each group. Histological and Biomechanical evaluation was performed to compare the differences in tendon-to-bone healing. Histology showed increased fibrocartilage, a clear boundary at the mineralized fibrocartilage, abundant collagen type III, and higher total scores, especially in the interposition group. GFP-overexpression was observed at the transplanted site at 2 weeks after repair. Although two groups where stem cell sheets applied showed higher load to failure than the repair-only group, the load to failure was not different between the interposition and overlay group. In the chronic rotator cuff repair model, stem cell sheets enhanced regeneration of the tendon-to-bone junction. This regeneration was effective when the stem cell sheet was interpositioned at the tendon-to-bone interface. Basic Science Study; In Vivo Animal Model; Histology and Biomechanics.