2D-shear wave elastography in the evaluation of suspicious superficial inguinal lymph nodes: Reproducibility and region of interest selection

• Published in: PloS one 2022-03, Vol.17 (3), p.e0265802-e0265802
• Main Authors: Lahtinen, Olli, Pulkkinen, Mika, Sironen, Reijo, Vanninen, Ritva, Rautiainen, Suvi
• Format: Article
• Language: English
• Subjects: Biology and Life Sciences; Biopsy; Cancer therapies; Clinical medicine; Correlation coefficient; Correlation coefficients; Diagnosis; Diagnostic imaging; Diameters; Elasticity; Elasticity Imaging Techniques - methods; Evaluation; Genital cancers; Humans; Lymph nodes; Lymph Nodes - diagnostic imaging; Lymphatic system; Medical diagnosis; Medical examination; Medicine; Medicine and Health Sciences; Metastases; Methods; Orthogonality; Pathology; Patients; Reproducibility; Reproducibility of Results; Research and Analysis Methods; Sensitivity; Sensitivity and Specificity; Stiffness
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0265802

To assess the ability of 2D-Shear wave elastography (2D-SWE) to evaluate its reproducibility, to define the optimal orientation and size of the region of interest (ROI), and to differentiate benign from malignant inguinal lymph nodes (LNs). Thirty-two suspicious inguinal LNs from 21 patients were evaluated with 2D-SWE. SWE measurements were obtained in two orthogonal planes. To investigate reproducibility, sensitivity and specificity, circular ROIs with a diameter of 1 mm, 2 mm, 3 mm and 5 mm were placed on the cortex of the LNs. Additionally, one freehand ROI was drawn covering majority of the LN. Two observers performed five sets of SWE measurements for each ROI size. All LNs underwent core needle biopsy or were surgically removed. The 3 mm ROI for Mean-E in axial plane showed high interrater agreement [intraclass correlation coefficient (ICC) 0.899] with the cut-off value of 7.31 kPa resulting in 88.9% sensitivity and 60.9% specificity for differentiating malignant from benign LNs. In benign LNs, mean elasticity of the ROI was lower (7.68 ± 3.82 kPa; range, 3.41-15.40 kPa) compared to the malignant LNs (15.81 ± 10.61 kPa; range, 3.86-36.45 kPa). The most reproducible way to measure stiffness in inguinal LNs is a 3 mm circular ROI centered on the cortex of the LN in axial plane. Elasticity values were higher in the malignant LNs reflecting the stiffer nature of the metastatic LNs. 2D-SWE offers a noninvasive ultrasonographic tool to assess superficial inguinal lymph nodes with high reproducibility.