The role of CDH2 and MCP-1 mRNAs of blood extracellular vesicles in predicting early-stage diabetic nephropathy

Extracellular vesicles (EVs), including exosomes and microvesicles, are involved in intercellular communication by transferring biomolecules such as mRNA, which has been shown to be as essential biomarkers for many physiological and pathological conditions such as diabetic nephropathy (DN). This stu...

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Bibliographic Details
Published in:PloS one 2022-04, Vol.17 (4), p.e0265619-e0265619
Main Authors: Dehghanbanadaki, Hojat, Forouzanfar, Katayoon, Kakaei, Ardeshir, Zeidi, Samaneh, Salehi, Negar, Arjmand, Babak, Razi, Farideh, Hashemi, Ehsan
Format: Article
Language:English
Subjects:
Antigens, CD - genetics
Biology and Life Sciences
Biomarkers
Biomolecules
Biopsy
Biotechnology
Blood
Cadherins
Cadherins - genetics
Cardiovascular disease
Cell organelles
Chemokine CCL2 - genetics
Creatinine
Development and progression
Diabetes
Diabetes mellitus
Diabetes Mellitus, Type 2
Diabetic nephropathies
Diabetic Nephropathies - diagnosis
Diabetic Nephropathies - genetics
Diabetic nephropathy
Diabetic retinopathy
Diagnosis
Diagnostic systems
E-cadherin
Endocrinology
Exosomes
Extracellular vesicles
Extracellular Vesicles - metabolism
Gene expression
Genetic aspects
Genetic engineering
Genomics
Health aspects
Health care
Humans
Medical laboratories
Medicine and Health Sciences
Messenger RNA
Metabolic disorders
Metabolites
Monocyte chemoattractant protein 1
Nephropathy
Physical Sciences
Plasma
Plasminogen Activator Inhibitor 1
Proteins
Proteinuria
RNA, Messenger - genetics
Urine
Vesicles
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Summary:Extracellular vesicles (EVs), including exosomes and microvesicles, are involved in intercellular communication by transferring biomolecules such as mRNA, which has been shown to be as essential biomarkers for many physiological and pathological conditions such as diabetic nephropathy (DN). This study aimed to investigate the expression of CDH1, CDH2, MCP-1, and PAI-1 mRNAs in blood EVs of DN patients and to determine their accuracy in predicting early-stage DN. We recruited 196 participants, including 35 overt DN patients, 53 incipient DN patients, 62 diabetic patients (DM), and 46 healthy individuals. Quantification of the mRNA profile of blood EVs was performed using the qRT-PCR method. The diagnostic performance of mRNA was evaluated using receiver operating characteristic analysis. The mRNA expression of CDH2 and MCP-1 was downregulated in overt DN group (0.22-fold change and 0.15-fold change, respectively) and incipient DN group (0.60-fold change and 0.43-fold change, respectively) compared to DM group (1.72-fold change and 2.77-fold change, respectively), while PAI-1 mRNA expression decreased in incipient DN group (0.70-fold change) and DM group (0.58-fold change) compared to control. However, the expression level of CDH1 mRNA was not significantly different among the four groups (p = 0.408). Moreover, CDH2 and MCP-1 mRNAs inversely correlated with creatinine (r = -0.370 and r = -0.361, p
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0265619