Soil bacterial assemblage responses to wildfire in low elevation southern California habitats

• Published in: PloS one 2022-04, Vol.17 (4), p.e0266256-e0266256
• Main Authors: Cox, Elena, Cavalcanti, André R O, Crane, 3rd, Edward J, Meyer, 3rd, Wallace M
• Format: Article
• Language: English
• Subjects: Bacteria; Bacteria - genetics; Biology; Biology and Life Sciences; California; Composition; Drought; Drought resistance; Ecology and Environmental Sciences; Ecosystem; Ecosystem biology; Ecosystem structure; Ecosystems; Elevation; Engineering and Technology; Environmental aspects; Fire resistance; Flowers & plants; Grasses; Grasslands; Habitats; Influence; Mountains; Plants; Prescribed fire; Recreation areas; Relative abundance; RNA, Ribosomal, 16S - genetics; rRNA 16S; Scrub; Soil; Soil bacteria; Soil microbiology; Soil microorganisms; Soils; Structure-function relationships; Wildfires
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0266256

Understanding how wildfires and modification in plant assemblages interact to influence soil bacteria assemblages is a crucial step in understanding how these disturbances may influence ecosystem structure and function. Here, we resampled soil from three study sites previously surveyed in spring 2016 and 2017 and compared soil bacterial assemblages prior to and six months after (spring 2019) the 2018 Woolsey Fire in the Santa Monica Mountain National Recreation Area using Illumina sequencing of the 16S rRNA gene. All sites harbored both native California sage scrub and a non-native (grassland or forbland) habitat, allowing us to examine how fire influenced bacterial assemblages in common southern California habitats. Most results contrasted with our a-priori hypotheses: (1) richness and diversity increased following the fire, (2) heat/drought resistant and sensitive bacteria did not show consistent and differing patterns by increasing and decreasing, respectively, in relative abundance after the fire, and (3) bacterial assemblage structure was only minimally impacted by fire, with no differences being found between 2017 (pre-fire) and 2019 (post-fire) in three of the six habitats sampled. As sage scrub and non-native grasslands consistently harbored unique bacterial assemblages both before and following the fire, modifications in plant compositions will likely have legacy effects on these soils that persist even after a fire. Combined, our results demonstrate that bacterial assemblages in southern California habitats are minimally affected by fire. Because direct impacts of fire are limited, but indirect impacts, e.g., modifications in plant compositions, are significant, plant restoration efforts following a fire should strive to revegetate sage scrub areas to prevent legacy changes in bacterial composition.