Survival and inactivation kinetics of Salmonella enterica serovar Typhimurium in irradiated and natural poultry litter microcosms

• Published in: PloS one 2022-04, Vol.17 (4), p.e0267178-e0267178
• Main Authors: Gutierrez, Alan, Schneider, Keith R
• Format: Article
• Language: English
• Subjects: Agricultural wastes; Ammonia; Animals; Bedding; Biology and Life Sciences; Contamination; Deactivation; Environmental conditions; Health aspects; Inactivation; Kinetics; Litter; Management; Manures; Medicine and Health Sciences; Microbiota (Symbiotic organisms); Microcosms; Microflora; Microorganisms; Pathogens; pH effects; Physical Sciences; Populations; Poultry; Poultry industry; Prevention; Research and Analysis Methods; Salmonella; Salmonella enterica; Salmonella food poisoning; Salmonella typhimurium; Serogroup; Soil; Soil amendment; Soil contamination; Survival; Water activity
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0267178

The use of poultry litter as a biological soil amendment presents a risk for the preharvest contamination of fresh produce by Salmonella. In order to properly assess this risk, it is important to understand the factors influencing the persistence of Salmonella in poultry litter. This research was performed to investigate the influence of indigenous microflora on the survival of Salmonella Typhimurium in poultry litter. Microcosms of irradiated (sterilized) and natural poultry litter were inoculated with S. Typhimurium, adjusted to pH 8.0, 0.92 water activity (aw), and stored at 30°C for 6 days. S. Typhimurium populations (log CFU g-1) declined in both litter treatments and there were no significant differences (P > 0.05) in recovery between litter treatments on any sampling days (0 to 6). The pH of the natural litter significantly increased (P < 0.05) from 8.42 on day 0 to 9.00 on day 6. By day 6, S. Typhimurium populations in both litter treatments fell below the limit of detection (1 log CFU g-1). The inactivation kinetics of S. Typhimurium in both litter treatments were described by the Weibull model. Under the experimental conditions (pH 8.0, 0.92 aw, 30°C), the presence or absence of poultry litter microflora did not significantly influence the survival of S. Typhimurium. This study demonstrates that the mere presence of poultry litter microflora will not inhibit Salmonella survival. Instead, inhibitory interactions between various microorganisms in litter and Salmonella are likely dependent on more favorable environmental conditions (e.g., aw, pH) for growth and competition.