Early rhythmicity in the fetal suprachiasmatic nuclei in response to maternal signals detected by omics approach

The suprachiasmatic nuclei (SCN) of the hypothalamus harbor the central clock of the circadian system, which gradually matures during the perinatal period. In this study, time-resolved transcriptomic and proteomic approaches were used to describe fetal SCN tissue-level rhythms before rhythms in cloc...

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Bibliographic Details
Published in:PLoS biology 2022-05, Vol.20 (5), p.e3001637-e3001637
Main Authors: Greiner, Philipp, Houdek, Pavel, Sládek, Martin, Sumová, Alena
Format: Article
Language:English
Subjects:
Analysis
Animals
Biology and Life Sciences
Cell signaling
Circadian Rhythm - genetics
Circadian rhythms
Clock gene
Darkness
Female
Fetus - physiology
Fetuses
Food
Food availability
Food selection
Gene expression
Genes
Genetic aspects
Histology
Hypothalamus
Medicine and Health Sciences
Mice
Population
Pregnancy
Proteomics
Rats
Research and Analysis Methods
Rhythm
Signaling
Suprachiasmatic nucleus
Suprachiasmatic Nucleus - metabolism
Surgery
Transcriptomics
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Summary:The suprachiasmatic nuclei (SCN) of the hypothalamus harbor the central clock of the circadian system, which gradually matures during the perinatal period. In this study, time-resolved transcriptomic and proteomic approaches were used to describe fetal SCN tissue-level rhythms before rhythms in clock gene expression develop. Pregnant rats were maintained in constant darkness and had intact SCN, or their SCN were lesioned and behavioral rhythm was imposed by temporal restriction of food availability. Model-selecting tools dryR and CompareRhythms identified sets of genes in the fetal SCN that were rhythmic in the absence of the fetal canonical clock. Subsets of rhythmically expressed genes were assigned to groups of fetuses from mothers with either intact or lesioned SCN, or both groups. Enrichment analysis for GO terms and signaling pathways revealed that neurodevelopment and cell-to-cell signaling were significantly enriched within the subsets of genes that were rhythmic in response to distinct maternal signals. The findings discovered a previously unexpected breadth of rhythmicity in the fetal SCN at a developmental stage when the canonical clock has not yet developed at the tissue level and thus likely represents responses to rhythmic maternal signals.
ISSN:1545-7885
1544-9173
1545-7885
DOI:10.1371/journal.pbio.3001637