LINC00922 promotes deterioration of gastric cancer

• Published in: PloS one 2022-05, Vol.17 (5), p.e0267798-e0267798
• Main Authors: Ge, Hua, Yan, Yan, Tian, Fei, Guo, Lingfei, He, Xueyan, Wang, Can, Song, Jiacheng, Deng, Zhilong
• Format: Article
• Language: English
• Subjects: Analysis; Apoptosis; Assaying; Bioinformatics; Biological activity; Biology and life sciences; Biomarkers; Cancer; Cell adhesion; Cell adhesion & migration; Cell cycle; Cell growth; Cell Line, Tumor; Cell migration; Cell proliferation; Cell Proliferation - genetics; Computer and Information Sciences; Diagnosis; Disease; Encyclopedias; Flow cytometry; Follistatin-Related Proteins - genetics; Gastric cancer; Gene expression; Gene Expression Regulation, Neoplastic; Genes; Genetic aspects; Genomes; Humans; Liver cancer; Medical prognosis; Medicine and Health Sciences; Metastasis; Prognosis; Protein interaction; Protein-protein interactions; Proteins; RNA; RNA, Long Noncoding - genetics; RNA, Long Noncoding - metabolism; Stomach cancer; Stomach Neoplasms - genetics; Stomach Neoplasms - metabolism; Survival; Tissues; Tumors; Wound healing
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0267798

Several studies have demonstrated the association of lncRNAs with a variety of cancers. Here, we explored the role of LINC00922 in gastric cancer (GC) using bioinformatics approaches and in vitro experiments. We examined the expression of LINC00922 and the prognosis of GC patients based on data from The Cancer Genome Atlas (TCGA) and Gene Expression Profiling Interactive Analysis (GEPIA). LINC00922-related genes were identified by the Multi Experiment Matrix (MEM) database and The Atlas of Noncoding RNAs in Cancer (TANRIC), followed by Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG) and protein-protein interaction analysis. The significance of LINC00922 in cell proliferation, apoptosis, invasion and migration was assessed by MTT assay, flow cytometry, Transwell assay and wound-healing assay. The expression of LINC00922 was increased in GC tissues compared with adjacent non-tumor tissues, and increased LINC00922 expression was correlated with poor overall survival and disease-free survival. In addition, 336 overlapping genes were identified by the MEM database and TANRIC and found to be involved in GC-related biological processes, such as cell adhesion and migration, as well as TGF-β signaling. In the protein-protein interaction network, hub genes, such as FSTL3 and LAMC1, were identified. LINC00922 overexpression significantly promoted cell proliferation and invasion in vitro, whereas LINC00922 knockdown exerted opposite effects. In summary, our findings indicate that LINC00922 is overexpressed in GC tissues, suggesting that it might play a role in the development and progression of GC, and thus, it might serve as a prognostic indicator of GC.