Experimental evaluation of a metofluthrin passive emanator against Aedes albopictus

• Published in: PloS one 2022-05, Vol.17 (5), p.e0267278-e0267278
• Main Authors: Zarella, Olivia, Ekwomadu, Uche, Romer, Yamila, Kirstein, Oscar D, Che-Mendoza, Azael, González-Olvera, Gabriela, Manrique-Saide, Pablo, Devine, Gregor, Vazquez-Prokopec, Gonzalo M
• Format: Article
• Language: English
• Subjects: Aedes - physiology; Aedes aegypti; Aedes albopictus; Animals; Aquatic insects; Biology and Life Sciences; Culicidae; Cyclopropanes; Data collection; Dengue fever; Disease transmission; Earth Sciences; Emission; Finite element method; Fluorobenzenes; Humans; Indoor environments; Insecticides; Insecticides - pharmacology; Laboratories; Medicine and Health Sciences; Mosquito Control; Mosquito Vectors; Mosquitoes; Outdoors; Paralysis; Polyethylene; Polyethylenes; Social Sciences; Tents; Urban areas; Urban environments; Vaccines; Viruses; Zika virus
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0267278

With the recent occurrence of locally transmitted Aedes-borne viruses in the continental United States and Europe, and a lack of effective vaccines, new approaches to control Aedes aegypti and Aedes albopictus are needed. In sub-tropical urban settings in the US, Ae. albopictus is a dominant nuisance and arbovirus vector species. Unfortunately, the vector control toolbox against Ae. albopictus is not as well developed as for Ae. aegypti. Here, we evaluate the efficacy, longevity, and range of protectiveness of a novel passive metofluthrin emanator (10% active ingredient in a polyethylene mesh) against Ae. albopictus indoors and outdoors. Four studies were conducted comparing the presence of the metofluthrin emanator to a control lacking emanator with interest in quantifying efficacy by human landing counts. Studies evaluated the effect of an emanator at varying distances from one or more human volunteers indoors and outdoors. Efficacy of emanators over time since activation was also evaluated. Mixed-effects models determined that sitting in close proximity to an emanator reduced landings by 89.5% outdoors and by 74.6% indoors. The emanator was determined protective when located immediately next to a human volunteer outdoors but not uniformly protective when located further away. The emanator was protective at all tested distances from the device indoors. Mortality of mosquitoes exposed to metofluthrin emanators was ~2x higher than those who were not exposed in indoor conditions. Finally, a Generalized Additive Model determined that emanators used continuously outdoors lost their effect after 2.5 weeks and stopped inducing paralysis in mosquitoes after 3.8 weeks of use. We show strong and lasting efficacy of 10% metofluthrin emanators against field Ae. albopictus both in indoor and outdoor conditions. Metofluthrin emanators can protect people from Ae. albopictus bites, representing a viable option for reducing human-mosquito contacts at home and beyond.