Bombyx mori Vps13d is a key gene affecting silk yield

Bombyx mori is an important economic insect, its economic value mainly reflected in the silk yield. The major functional genes affecting the silk yield of B. mori have not been determined yet. Bombyx mori vacuolar protein sorting-associated protein 13d (BmVps13d) has been identified, but its functio...

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Bibliographic Details
Published in:PloS one 2022-07, Vol.17 (7), p.e0270840-e0270840
Main Authors: Zhao, Luochao, Sun, Xia, Wang, Xueyang, Qin, Sheng, Kong, Yunhui, Li, Muwang
Format: Article
Language:English
Subjects:
Adenine
Biology and Life Sciences
Bombyx mori
CRISPR
Economics
Genes
Genetic aspects
Genomes
Insects
Larvae
Metabolism
Midgut
Mutation
Nucleotides
Protein transport
Proteins
Research and analysis methods
Ribonucleotides
Silk
Silk gland
Silkworms
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Summary:Bombyx mori is an important economic insect, its economic value mainly reflected in the silk yield. The major functional genes affecting the silk yield of B. mori have not been determined yet. Bombyx mori vacuolar protein sorting-associated protein 13d (BmVps13d) has been identified, but its function is not reported. In this study, BmVps13d protein shared 30.84% and 34.35% identity with that of in Drosophila melanogaster and Homo. sapiens, respectively. The expressions of BmVps13d were significantly higher in the midgut and silk gland of JS (high silk yield) than in that of L10 (low silk yield). An insertion of 9 bp nucleotides and two deficiencies of adenine ribonucleotides in the putative promoter region of BmVps13d gene in L10 resulted in the decline of promoter activity was confirmed using dual luciferase assay. Finally, the functions of BmVps13d in B. mori were studied using the CRISPR/Cas9 system, and the mutation of BmVps13d resulted in a 24.7% decline in weight of larvae, as well as a 27.1% (female) decline and a 11.8% (male) decline in the silk yield. This study provides a foundation for studying the molecular mechanism of silk yield and breeding the silkworm with high silk yield.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0270840