Mod3D: A low-cost, flexible modular system of live-cell microscopy chambers and holders

Live-cell microscopy imaging typically involves the use of high-quality glass-bottom chambers that allow cell culture, gaseous buffer exchange and optical properties suitable for microscopy applications. However, commercial sources of these chambers can add significant annual costs to cell biology l...

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Published in:PloS one 2022-06, Vol.17 (6), p.e0269345-e0269345
Main Authors: Goss, Siobhan, Barba Bazan, Carlos, Neuman, Kaitlyn, Peng, Christina, Begeja, Nola, Suart, Celeste Elisabeth, Truant, Ray
Format: Article
Language:English
Subjects:
3-D printers
Biodegradability
Biodegradation
Biology and Life Sciences
Bioplastics
Cell culture
Chambers
Consumer products
Costs
Curing
Design
Engineering and technology
Equipment and supplies
Lab Protocol
Laboratories
LCDs
Liquid crystal displays
Manufacturing
Medical laboratories
Medical research
Methods
Microscope and microscopy
Microscopy
Modular systems
Open source software
Optical properties
Physical Sciences
Plastic debris
Production methods
Prototyping
Research and Analysis Methods
Three dimensional printing
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container_end_page e0269345
container_issue 6
container_start_page e0269345
container_title PloS one
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creator Goss, Siobhan
Barba Bazan, Carlos
Neuman, Kaitlyn
Peng, Christina
Begeja, Nola
Suart, Celeste Elisabeth
Truant, Ray
description Live-cell microscopy imaging typically involves the use of high-quality glass-bottom chambers that allow cell culture, gaseous buffer exchange and optical properties suitable for microscopy applications. However, commercial sources of these chambers can add significant annual costs to cell biology laboratories. Consumer products in three-dimensional printing technology, for both Filament Deposition Modeling (FDM) and Masked Stereo Lithography (MSLA), have resulted in more biomedical research labs adopting the use of these devices for prototyping and manufacturing of lab plastic-based items, but rarely consumables. Here we describe a modular, live-cell chamber with multiple design options that can be mixed per experiment. Single reusable carriers and the use of biodegradable plastics, in a hybrid of FDM and MSLA manufacturing methods, reduce plastic waste. The system is easy to adapt to bespoke designs, with concept-to-prototype in a single day, offers significant cost savings to the users over commercial sources, and no loss in dimensional quality or reliability.
doi_str_mv 10.1371/journal.pone.0269345
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subjects 3-D printers
Biodegradability
Biodegradation
Biology and Life Sciences
Bioplastics
Cell culture
Chambers
Consumer products
Costs
Curing
Design
Engineering and technology
Equipment and supplies
Lab Protocol
Laboratories
LCDs
Liquid crystal displays
Manufacturing
Medical laboratories
Medical research
Methods
Microscope and microscopy
Microscopy
Modular systems
Open source software
Optical properties
Physical Sciences
Plastic debris
Production methods
Prototyping
Research and Analysis Methods
Three dimensional printing
title Mod3D: A low-cost, flexible modular system of live-cell microscopy chambers and holders
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