Pseudomonas aeruginosa Ld-08 isolated from Lilium davidii exhibits antifungal and growth-promoting properties

• Published in: PloS one 2022-06, Vol.17 (6), p.e0269640-e0269640
• Main Authors: Khan, Mohammad Sayyar, Gao, Junlian, Zhang, Mingfang, Xue, Jing, Zhang, Xiuhai
• Format: Article
• Language: English
• Subjects: Acetic acid; Acids; Agriculture; Analysis; Antifungal agents; Bacteria; Biological control; Biology and Life Sciences; Botryosphaeria; Bulbs; Disease; Endophytes; Enzymes; Ethyl acetate; Fungi; Fungicides; Fusarium fujikuroi; Fusarium oxysporum; Herbal medicine; Lilium davidii; Mass spectrometry; Medicine and Health Sciences; Metabolites; Morphology; Nocodazole; Organic acids; Oxolinic acid; Pathogens; Peptides; Physical Sciences; Plant bacterial diseases; Plant growth; Plant metabolites; Properties; Pseudomonas aeruginosa; Quinolones; rRNA 16S; Scientific imaging; Secondary metabolites; Sequence analysis; Solubilization; Solvents; Sustainable agriculture; Ursodeoxycholic acid; Venlafaxine
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0269640

A plant growth-promoting and antifungal endophytic bacteria designated as Ld-08 isolated from the bulbs of Lilium davidii was identified as Pseudomonas aeruginosa based on phenotypic, microscopic, and 16S rRNA gene sequence analysis. Ld-08 exhibited antifungal effects against Fusarium oxysporum, Botrytis cinerea, Botryosphaeria dothidea, and Fusarium fujikuroi. Ld-08 showed the highest growth inhibition, i.e., 83.82±4.76% against B. dothidea followed by 74.12±3.87%, 67.56±3.35%, and 63.67±3.39% against F. fujikuroi, B. cinerea, and F. oxysporum, respectively. The ethyl acetate fraction of Ld-08 revealed the presence of several bioactive secondary metabolites. Prominent compounds were quinolones; 3,9-dimethoxypterocarpan; cascaroside B; dehydroabietylamine; epiandrosterone; nocodazole; oxolinic acid; pyochelin; rhodotulic acid; 9,12-octadecadienoic acid; di-peptides; tri-peptides; ursodiol, and venlafaxine. The strain Ld-08 showed organic acids, ACC deaminase, phosphate solubilization, IAA, and siderophore. The sterilized bulbs of a Lilium variety, inoculated with Ld-08, were further studied for plant growth-promoting traits. The inoculated plants showed improved growth than the control plants. Importantly, some growth parameters such as plant height, leaf length, bulb weight, and root length were significantly (P [less than or equal to]0.05) increased in the inoculated plants than in the control un-inoculated plants. Further investigations are required to explore the potential of this strain to be used as a plant growth-promoting and biocontrol agent in sustainable agriculture.