Examining the association between the gastrointestinal microbiota and Gulf War illness: A prospective cohort study

• Published in: PloS one 2022-07, Vol.17 (7), p.e0268479-e0268479
• Main Authors: Kates, Ashley, Keating, Julie, Baubie, Kelsey, Putman-Buehler, Nathan, Watson, Lauren, Godfrey, Jared, Deblois, Courtney L, Suen, Garret, Cook, Dane B, Rabago, David, Gangnon, Ronald, Safdar, Nasia
• Format: Article
• Language: English
• Subjects: Analysis; Biology and Life Sciences; Care and treatment; Chronic fatigue syndrome; Cognitive ability; Cohort analysis; Diagnosis; Diet; Digestive system; Digestive tract; Diseases; Eating behavior; Gastrointestinal diseases; Gastrointestinal tract; Gulf War; Gulf War syndrome; Intestinal microflora; Irritable bowel syndrome; Longitudinal studies; Medicine and Health Sciences; Microbiomes; Microbiota; Microbiota (Symbiotic organisms); Pain; Patients; Persian Gulf War; Persian Gulf War veterans; Questionnaires; R&D; Research & development; Research and Analysis Methods; Risk factors; rRNA 16S; Ruminococcaceae; Signs and symptoms; Skin diseases; Statistical analysis; Taxonomy; War
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0268479

Gulf War Illness (GWI) affects 25–35% of the 1991 Gulf War Veteran (GWV) population. Patients with GWI experience pain, fatigue, cognitive impairments, gastrointestinal dysfunction, skin disorders, and respiratory issues. In longitudinal studies, many patients with GWI have shown little to no improvement in symptoms since diagnosis. The gut microbiome and diet play an important role in human health and disease, and preliminary studies suggest it may play a role in GWI. To examine the relationship between the gut microbiota, diet, and GWI, we conducted an eight-week prospective cohort study collecting stool samples, medications, health history, and dietary data. Sixty-nine participants were enrolled into the study, 36 of which met the case definition for GWI. The gut microbiota of participants, determined by 16S rRNA sequencing of stool samples, was stable over the duration of the study and showed no within person (alpha diversity) differences. Between group analyses (beta diversity) identified statistically significant different between those with and without GWI. Several taxonomic lineages were identified as differentially abundant between those with and without GWI (n = 9) including a greater abundance of Lachnospiraceae and Ruminococcaceae in those without GWI. Additionally, there were taxonomic differences between those with high and low healthy eating index (HEI) scores including a greater abundance of Ruminococcaceae in those with higher HEI scores. This longitudinal cohort study of GWVs found that participants with GWI had significantly different microbiomes from those without GWI. Further studies are needed to determine the role these differences may play in the development and treatment of GWI.