The potential role of serum expression profile of long non coding RNAs, Cox2 and HOTAIR as novel diagnostic biomarkers in systemic lupus erythematosus

The role of the long non-coding RNAs (lncRNAs) in the pathogenesis of systemic lupus erythematosus (SLE) is mostly unknown, despite increasing evidence that lncRNAs extensively participate in physiological and pathological conditions. To detect the level of lncRNA-Cox2, HOTAIR, IL-6, and MMP-9 in th...

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Published in:PloS one 2022-08, Vol.17 (8), p.e0268176
Main Authors: Mahmoud, Rania H, Fouad, Nermeen A, Hefzy, Enas M, Shaker, Olfat G, Ahmed, Tarek I, Hussein, Hoda A, Nasr, Maha H, Zaki, Othman M, Abdelghaffar, Noha K, Abdelaleem, Omayma O
Format: Article
Language:English
Subjects:
Analysis
Antibodies
Autoimmune diseases
Biological markers
Biology and Life Sciences
Biomarkers
Chronic conditions
Coding
Cyclooxygenase-2
Cytokines
Diagnosis
Enzyme-linked immunosorbent assay
Gelatinase B
Gene expression
Genetic aspects
Humans
Inflammatory diseases
Interleukin 6
Interleukin-6 - blood
Laboratories
Lupus
Lupus Erythematosus, Systemic - diagnosis
Lupus Erythematosus, Systemic - genetics
Lupus Nephritis
Matrix Metalloproteinase 9 - blood
Medical diagnosis
Medicine and Health Sciences
Nephritis
Non-coding RNA
Pathogenesis
Patients
Polymerase chain reaction
Proteins
Proteinuria
Regression analysis
Rheumatology
RNA
RNA, Long Noncoding - genetics
Sample size
Sensitivity
Serum levels
Systemic lupus erythematosus
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Summary:The role of the long non-coding RNAs (lncRNAs) in the pathogenesis of systemic lupus erythematosus (SLE) is mostly unknown, despite increasing evidence that lncRNAs extensively participate in physiological and pathological conditions. To detect the level of lncRNA-Cox2, HOTAIR, IL-6, and MMP-9 in the serum of SLE patients and to correlate these levels with disease activity and patients' clinical and laboratory data to evaluate the value of these biomarkers for SLE diagnosis and assessment of disease activity. Blood samples from 58 SLE patients, and 60 healthy controls (HCs) were used for detection of lncRNAs-Cox2 and HOTAIR expression levels by real-time polymerase chain reaction. Both IL-6 and MMP-9 serum levels were assayed by enzyme-linked immunosorbent assay. Lupus activity was assessed with the Systemic Lupus Erythematosus Disease Activity Index (SLEDAI). The serum expression levels of lncRNA-Cox2 and HOTAIR were significantly up-regulated in SLE patients vs HCs (fold change [median (IQR) was 1.29(0.81-1.71, P
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0268176