Distinct functions of three chromatin remodelers in activator binding and preinitiation complex assembly

The nucleosome remodeling complexes (CRs) SWI/SNF, RSC, and Ino80C cooperate in evicting or repositioning nucleosomes to produce nucleosome depleted regions (NDRs) at the promoters of many yeast genes induced by amino acid starvation. We analyzed mutants depleted of the catalytic subunits of these C...

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Bibliographic Details
Published in:PLoS genetics 2022-07, Vol.18 (7), p.e1010277-e1010277
Main Authors: Rawal, Yashpal, Qiu, Hongfang, Hinnebusch, Alan G
Format: Article
Language:English
Subjects:
Amino acid starvation
Amino acids
Analysis
Binding sites
Biology and Life Sciences
Catalytic subunits
Cell division
Chromatin
Cooperation
Evictions
Gene expression
Gene mutations
Genetic research
Genomes
Metabolism
Nucleosomes
Promoters
Protein binding
Research and Analysis Methods
RNA polymerase
TATA-binding protein
Yeast
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Summary:The nucleosome remodeling complexes (CRs) SWI/SNF, RSC, and Ino80C cooperate in evicting or repositioning nucleosomes to produce nucleosome depleted regions (NDRs) at the promoters of many yeast genes induced by amino acid starvation. We analyzed mutants depleted of the catalytic subunits of these CRs for binding of transcriptional activator Gcn4 and recruitment of TATA-binding protein (TBP) during preinitiation complex (PIC) assembly. RSC and Ino80 were found to enhance Gcn4 binding to both UAS elements in NDRs upstream of promoters and to unconventional binding sites within nucleosome-occupied coding sequences; and SWI/SNF contributes to UAS binding when RSC is depleted. All three CRs are actively recruited by Gcn4 to most UAS elements and appear to enhance Gcn4 binding by reducing nucleosome occupancies at the binding motifs, indicating a positive regulatory loop. SWI/SNF acts unexpectedly in WT cells to prevent excessive Gcn4 binding at many UAS elements, indicating a dual mode of action that is modulated by the presence of RSC. RSC and SWI/SNF collaborate to enhance TBP recruitment at Gcn4 target genes, together with Ino80C, in a manner associated with nucleosome eviction at the TBP binding sites. Cooperation among the CRs in TBP recruitment is also evident at the highly transcribed ribosomal protein genes, while RSC and Ino80C act more broadly than SWI/SNF at the majority of other constitutively expressed genes to stimulate this step in PIC assembly. Our findings indicate a complex interplay among the CRs in evicting promoter nucleosomes to regulate activator binding and stimulate PIC assembly.
ISSN:1553-7404
1553-7390
1553-7404
DOI:10.1371/journal.pgen.1010277