Ectopic expression of WRINKLED1 in rice improves lipid biosynthesis but retards plant growth and development

WRINKLED1 (WRI1) is a transcription factor which is key to the regulation of seed oil biosynthesis in Arabidopsis. In the study, we identified two WRI1 genes in rice, named OsWRI1a and OsWRI1b, which share over 98% nucleotide similarity and are expressed only at very low levels in leaves and endospe...

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Published in:PloS one 2022-08, Vol.17 (8), p.e0267684-e0267684
Main Authors: Liu, Kaiqi, Tang, Yuehui, Tang, Yongyan, Li, Meiru, Wu, Guojiang, Chen, Yaping, Jiang, Huawu
Format: Article
Language:English
Subjects:
Amino acids
Angiosperms
Arabidopsis
Biology and Life Sciences
Biosynthesis
Cloning
Ectopic expression
Endosperm
Engineering and Technology
Enzymes
Fatty acids
Gene expression
Genes
Genetic aspects
Genetically modified plants
Glucose
Glycolysis
Growth
Laminates
Leaves
Lipids
Localization
Nucleotides
Oils & fats
Phenotypes
Phylogenetics
Plant growth
Properties
Proteins
Protoplasts
Research and Analysis Methods
Rice
Seeds
Transcription factors
Transgenic plants
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Summary:WRINKLED1 (WRI1) is a transcription factor which is key to the regulation of seed oil biosynthesis in Arabidopsis. In the study, we identified two WRI1 genes in rice, named OsWRI1a and OsWRI1b, which share over 98% nucleotide similarity and are expressed only at very low levels in leaves and endosperms. The subcellular localization of Arabidopsis protoplasts showed that OsWRI1a encoded a nuclear localized protein. Overexpression of OsWRI1a under the control of the CaMV 35S promoter severely retarded plant growth and development in rice. Expressing the OsWRI1a gene under the control of the P1 promoter of Brittle2 (highly expressed in endosperm but low in leaves and roots) increased the oil content of both leaves and endosperms and upregulated the expression of several genes related to late glycolysis and fatty acid biosynthesis. However, the growth and development of the transgenic plants were also affected, with phenotypes including smaller plant size, later heading time, and fewer and lighter grains. The laminae (especially those of flag leaves) did not turn green and could not unroll normally. Thus, ectopic expression of OsWRI1a in rice enhances oil biosynthesis, but also leads to abnormal plant growth and development.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0267684