Integrated genome-based assessment of safety and probiotic characteristics of Lactiplantibacillus plantarum PMO 08 isolated from kimchi

• Published in: PloS one 2022-10, Vol.17 (10), p.e0273986-e0273986
• Main Authors: Oh, Young Joo, Kim, Seul-Ah, Yang, Soo Hwi, Kim, Da Hye, Cheng, Ya-Yun, Kang, Jung Il, Lee, Sang Yun, Han, Nam Soo
• Format: Article
• Language: English
• Subjects: Ampicillin; Antibiotic resistance; Antibiotics; Antimicrobial agents; Beverages; Bile; Biology and Life Sciences; Carbohydrates; Cholesterol; Chromosomes; Drug resistance; Engineering and Technology; Factor analysis; Fermentation; Fermented food; Food; Gene sequencing; Genes; Genomes; Health aspects; Health promotion; In vitro methods and tests; Inflammation; Inositols; Kimchi; Lactiplantibacillus plantarum; Lactobacillus; Medicine and Health Sciences; myo-Inositol-1 (or 4)-monophosphatase; Nucleotide sequence; Nucleotides; Oxidative stress; Phenotypes; Physical Sciences; Plasmids; Probiotics; Safety; Starter cultures; Taxonomy; Virulence; Virulence factors
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0273986

Lactiplantibacillus plantarum PMO 08 has been used as a probiotic starter culture for plant-based fermented beverages, with various health-promoting effects such as cholesterol-lowering and anti-inflammatory activities. This study aimed to analyze the genome sequence of Lp. plantarum PMO 08 and identify its safety and probiotic characteristics at the genomic level. For this, complete genome sequencing was conducted to investigate the genes associated with risk and probiotic characteristics by using Pacbio combined with Illumina HiSeq. This bacterial strain has one circular chromosome of 3,247,789 bp with 44.5% G + C content and two plasmids of 50,296 bp with 39.0% G + C content and 19,592 bp with 40.5% G + C content. Orthologous average nucleotide identity analysis showed that PMO 08 belongs to the Lp. plantarum group with 99.14% similarity to Lp. plantarum WCFS1. No deleterious genes were determined in the virulence factor analysis, and no hemolysin activity or secondary bile salt synthesis were detected in vitro test. In the case of antibiotic resistance analysis, PMO 08 was resistant to ampicillin in vitro test, but these genes were not transferable. In addition, the strain showed same carbohydrate utilization with Lp. plantarum WCFS1, except for mannopyranoside, which only our strain can metabolize. The strain also harbors a gene for inositol monophosphatase family protein related with phytate hydrolysis and have several genes for metabolizing various carbohydrate which were rich in plant environment. Furthermore, in probiotic characteristics several genes involved in phenotypes such as acid/bile tolerance, adhesion ability, and oxidative stress response were detected in genome analysis. This study demonstrates that Lp. plantarum PMO 08 harbors several probiotic-related genes (with no deleterious genes) and is a suitable probiotic starter for plant-based fermentation.