A tRNA-derived fragment present in E. coli OMVs regulates host cell gene expression and proliferation

RNA-sequencing has led to a spectacular increase in the repertoire of bacterial sRNAs and improved our understanding of their biological functions. Bacterial sRNAs have also been found in outer membrane vesicles (OMVs), raising questions about their potential involvement in bacteria-host relationshi...

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Bibliographic Details
Published in:PLoS pathogens 2022-09, Vol.18 (9), p.e1010827-e1010827
Main Authors: Diallo, Idrissa, Ho, Jeffrey, Lambert, Marine, Benmoussa, Abderrahim, Husseini, Zeinab, Lalaouna, David, Massé, Eric, Provost, Patrick
Format: Article
Language:English
Subjects:
Bacteria
Biochemistry, Molecular Biology
Biology and life sciences
Biosynthesis
Cell proliferation
E coli
Environmental stress
Escherichia coli
Gene expression
Gene sequencing
Genetic aspects
Health aspects
Kinases
Life Sciences
Medicine and Health Sciences
Membrane vesicles
Molecular biology
Physical Sciences
Research and Analysis Methods
Ribonucleic acid
RNA
RNA sequencing
Statistical analysis
Symbiosis
Transfer RNA
tRNA
Variance analysis
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Summary:RNA-sequencing has led to a spectacular increase in the repertoire of bacterial sRNAs and improved our understanding of their biological functions. Bacterial sRNAs have also been found in outer membrane vesicles (OMVs), raising questions about their potential involvement in bacteria-host relationship, but few studies have documented this issue. Recent RNA-Sequencing analyses of bacterial RNA unveiled the existence of abundant very small RNAs (vsRNAs) shorter than 16 nt. These especially include tRNA fragments (tRFs) that are selectively loaded in OMVs and are predicted to target host mRNAs. Here, in Escherichia coli (E. coli), we report the existence of an abundant vsRNA, Ile-tRF-5X, which is selectively modulated by environmental stress, while remaining unaffected by inhibition of transcription or translation. Ile-tRF-5X is released through OMVs and can be transferred to human HCT116 cells, where it promoted MAP3K4 expression. Our findings provide a novel perspective and paradigm on the existing symbiosis between bacteria and human cells.
ISSN:1553-7374
1553-7366
1553-7374
DOI:10.1371/journal.ppat.1010827