In vitro versus cryo-induced capacitation of bovine spermatozoa, part 1: Structural, functional, and oxidative similarities and differences

Low temperatures during cryopreservation activate a cascade of changes, which may lead into irreversible damage and reduction of the fertilization potential, including the process of premature capacitation. The aim of our study was to evaluate the range of cell damage following the cryopreservation...

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Bibliographic Details
Published in:PloS one 2022-10, Vol.17 (10), p.e0276683
Main Authors: Benko, Filip, Mohammadi-Sangcheshmeh, Abdollah, Ďuračka, Michal, Lukáč, Norbert, Tvrdá, Eva
Format: Article
Language:English
Subjects:
Acrosome reaction
Adenosine monophosphate
Adenosine Monophosphate - metabolism
Animals
Biology and Life Sciences
Capacitation
Cattle
Cholesterol
Cryopreservation
Cryopreservation - veterinary
Cryopreservation of organs, tissues, etc
Cyclic AMP
Damage
Deoxyribonucleic acid
DNA
DNA fragmentation
Fatty acids
Fertilization
Kinases
Laboratories
Low temperature
Male
Membrane potential
Membranes
Methods
Mitochondria
Motility
Nitrogen
Oxidative Stress
Oxygen
Parameters
Physical Sciences
Physiological aspects
Physiology
Reactive oxygen species
Reactive Oxygen Species - metabolism
Research and Analysis Methods
Semen
Semen Preservation - veterinary
Sodium
Sperm
Sperm Capacitation - physiology
Sperm Motility - physiology
Spermatozoa
Spermatozoa - metabolism
Structural integrity
Structure
Structure-function relationships
Superoxides - metabolism
Thermal properties
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Summary:Low temperatures during cryopreservation activate a cascade of changes, which may lead into irreversible damage and reduction of the fertilization potential, including the process of premature capacitation. The aim of our study was to evaluate the range of cell damage following the cryopreservation process and possible activation of cryocapacitation in bovine spermatozoa. For the experiments semen samples were obtained from 30 sexually mature Holstein bulls. Within the analysed parameters, we focused on the functional activity, structural integrity, capacitation status and oxidative profile. The samples were divided into three experimental groups, control (CTRL), in vitro capacitated (CAP) and cryopreserved (CRYO). Based on the collected data, there was a significant decrease in the sperm motility, mitochondrial membrane potential and concentration of cyclic adenosine monophosphate in the CRYO group when compared to CAP and CTRL (P
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0276683