Comparative diagnostic accuracy between simplified and original flow cytometric gating strategies for peripheral blood neutrophil myeloperoxidase expression in ruling out myelodysplastic syndromes

• Published in: PloS one 2022-11, Vol.17 (11), p.e0276095-e0276095
• Main Authors: Raskovalova, Tatiana, Scheffen, Laura, Jacob, Marie-Christine, Vettier, Claire, Bulabois, Bénédicte, Szymanski, Gautier, Chevalier, Simon, Gonnet, Nicolas, Park, Sophie, Labarère, José
• Format: Article
• Language: English
• Subjects: Accuracy; Biology and Life Sciences; Blood; Bone marrow; Boolean; Coefficient of variation; Confidence intervals; Correlation coefficient; Correlation coefficients; Diagnosis; Diagnostic systems; Disorders; Flow cytometry; Gating; Granulocytes; Health aspects; Leukocytes (neutrophilic); Lymphocytes; Measurement; Medical diagnosis; Medical examination; Medicine and Health Sciences; Methods; Myelodysplastic syndrome; Myelodysplastic syndromes; Neutrophils; Patients; Peripheral blood; Peroxidase; Research and Analysis Methods; Resource utilization; Validation studies; Vortices
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0276095

Flow cytometric analysis of peripheral blood neutrophil myeloperoxidase expression is accurate in ruling out myelodyplastic syndromes (MDS) but might not be suitable for implementation in busy clinical laboratories. We aimed to simplify the original gating strategy and examine its accuracy. Using the individual data from 62 consecutive participants enrolled in a prospective validation study, we assessed the agreement in intra-individual robust coefficient of variation (RCV) of peripheral blood neutrophil myeloperoxidase expression and compared diagnostic accuracy between the simplified and original gating strategies. Cytomorphological evaluation of bone marrow aspirate confirmed MDS in 23 patients (prevalence, 37%), unconfirmed MDS in 32 patients (52%), and was uninterpretable in 7 patients (11%). Median intra-individual RCV for simplified and original gating strategies were 30.7% (range, 24.7-54.4) and 30.6% (range, 24.7-54.1), with intra-class correlation coefficient quantifying absolute agreement equal to 1.00 (95% confidence interval [CI], 0.99 to 1.00). The areas under the receiver operating characteristic (ROC) curves were 0.93 (95% CI, 0.82-0.98) and 0.92 (95% CI, 0.82-0.98), respectively (P = .32). Using simplified or original gating strategy, intra-individual RCV values lower than a pre-specified threshold of 30.0% ruled out MDS for 35% (19 of 55) patients, with both sensitivity and negative predictive value estimates of 100%. The simplified gating strategy performs as well as the original one for ruling out MDS and has the potential to save time and reduce resource utilization. Yet, prospective validation of the simplified gating strategy is warranted before its adoption in routine.