A model for microbial interactions and metabolomic alterations in Candida glabrata-Staphylococcus epidermidis dual-species biofilms

• Published in: PloS one 2022-12, Vol.17 (12), p.e0279069-e0279069
• Main Authors: Salvatore, Maria Michela, Maione, Angela, La Pietra, Alessandra, Carraturo, Federica, Staropoli, Alessia, Vinale, Francesco, Andolfi, Anna, Salvatore, Francesco, Guida, Marco, Galdiero, Emilia
• Format: Article
• Language: English
• Subjects: Antifungal activity; Antifungal agents; Antifungal Agents - metabolism; Antifungal Agents - pharmacology; Antimicrobial resistance; Biofilms; Biology and Life Sciences; Biomass; Candida; Candida albicans; Candida glabrata; Discriminant analysis; Drug resistance; Experiments; Extracellular matrix; Fungicides; Gene expression; Genes; Glucose; Humans; Medical equipment; Medicine and Health Sciences; Metabolism; Metabolites; Metabolomics; Microbial Interactions; Microbial mats; Microorganisms; Nosocomial infection; Nosocomial infections; Opportunist infection; Pathogens; Physical Sciences; Properties; Research and Analysis Methods; Secondary metabolites; Species; Stains & staining; Staphylococcus; Staphylococcus epidermidis; Variance analysis; Virulence
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0279069

The fungus Candida glabrata and the bacterium Staphylococcus epidermidis are important biofilm-forming microorganisms responsible of nosocomial infections in patients. In addition to causing single-species disease, these microorganisms are also involved in polymicrobial infections leading to an increased antimicrobial resistance. To expand knowledge about polymicrobial biofilms, in this study we investigate the formation of single- and dual-species biofilms of these two opportunistic pathogens employing several complementary approaches. First, biofilm biomass, biofilm metabolic activity and the microbial composition in single- and dual-species biofilms were assessed and compared. Then, the expression of three genes of C. glabrata and three genes of S. epidermidis positively related to the process of biofilm formation was evaluated. Although S. epidermidis is a stronger biofilm producer than C. glabrata, both biological and genetic data indicate that S. epidermidis growth is inhibited by C. glabrata which dominates the dual-species biofilms. To better understand the mechanisms of the interactions between the two microorganisms, a broad GC-MS metabolomic dataset of extracellular metabolites for planktonic, single- and dual-species biofilm cultures of C. glabrata and S. epidermidis was collected. As demonstrated by Partial Least Squares Discriminant Analysis (PLS-DA) of GC-MS metabolomic data, planktonic cultures, single- and dual-species biofilms can be sharply differentiated from each other by the nature and levels of an assortment of primary and secondary metabolites secreted in the culture medium. However, according to our data, 2-phenylethanol (secreted by C. glabrata) and the synergistically combined antifungal activity of 3-phenyllactic acid and of the cyclic dipeptide cyclo-(l-Pro-l-Trp) (secreted by S. epidermidis) play a major role in the race of the two microorganisms for predominance and survival.