Rebamipide treatment ameliorates obesity phenotype by regulation of immune cells and adipocytes

• Published in: PloS one 2022-12, Vol.17 (12), p.e0277692-e0277692
• Main Authors: Jhun, JooYeon, Moon, Jeonghyeon, Kim, Se-Young, Cho, Keun-Hyung, Na, Hyun Sik, Choi, JeongWon, Jung, Yoon Ju, Song, Kyo Young, Min, Jun-Ki, Cho, Mi-La
• Format: Article
• Language: English
• Subjects: 3T3-L1 Cells; Abnormalities; Adipocytes; Adipocytes - metabolism; Adipose tissue; Adipose Tissue - metabolism; Amino acids; Analysis; Animals; Antibodies; Arthritis; Bioaccumulation; Biology and Life Sciences; Blood glucose; Body fat; Body weight; Body weight gain; Care and treatment; Cell differentiation; Cell lines; Cholesterol; Cytokines; Diabetes; Diet; Diet, High-Fat - adverse effects; Glucose; Helper cells; High fat diet; Immune system; Inflammation; Inflammation - metabolism; Insulin; Insulin Resistance; Lipid metabolism; Lipids; Lipoproteins; Lymphocytes T; Macrophages; Medicine and Health Sciences; Metabolism; Mice; Mice, Inbred C57BL; Obesity; Obesity - complications; Osteoarthritis; Oxidative stress; Patient outcomes; Phenotype; Phenotypes; Quinolones - metabolism; Quinolones - pharmacology; Quinolones - therapeutic use; Regulation; Research and Analysis Methods; Research ethics; Statistical analysis; Tumor necrosis factor-TNF
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0277692

Obesity is a medical term used to describe an over-accumulation of adipose tissue. It causes abnormal physiological and pathological processes in the body. Obesity is associated with systemic inflammation and abnormalities in immune cell function. Rebamipide, an amino acid derivative of 2-(1H)-quinolinone, has been used as a therapeutic for the protection from mucosal damage. Our previous studies have demonstrated that rebamipide treatment regulates lipid metabolism and inflammation, leading to prevention of weight gain in high-fat diet mice. In this study, mice were put on a high calorie diet for 11 weeks while receiving injections of rebamipide. Rebamipide treatment reduced the body weight, liver weight and blood glucose levels compared to control mice and reduced both glucose and insulin resistance. Fat accumulation has been shown to cause pro-inflammatory activity in mice. Treatment with rebamipide decreased the prevalence of inflammatory cells such as Th2, Th17 and M1 macrophages and increased anti-inflammatory Treg and M2 macrophages in epididymal fat tissue. Additionally, rebamipide addition inhibited adipocyte differentiation in 3T3-L1 cell lines. Taken together, our study demonstrates that rebamipide treatment is a novel and effective method to prevent diet-induced obesity.