Development of a simple, rapid, and sensitive diagnostic assay for enterotoxigenic E. coli and Shigella spp applicable to endemic countries

Enterotoxigenic E. coli (ETEC) and Shigella spp (Shigella) are complex pathogens. The diagnostic assays currently used to detect these pathogens are elaborate or complicated, which make them difficult to apply in resource poor settings where these diseases are endemic. The culture methods used to de...

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Bibliographic Details
Published in:PLoS neglected tropical diseases 2022-01, Vol.16 (1), p.e0010180-e0010180
Main Authors: Chakraborty, Subhra, Connor, Sean, Velagic, Mirza
Format: Article
Language:English
Subjects:
Assaying
Biology and Life Sciences
Detection
Diagnostic systems
Diagnostic tests
DNA, Bacterial - analysis
Dysentery, Bacillary - diagnosis
Dysentery, Bacillary - microbiology
E coli
Earth Sciences
Enterotoxigenic Escherichia coli - genetics
Enterotoxigenic Escherichia coli - isolation & purification
Escherichia coli
Escherichia coli Infections - diagnosis
Escherichia coli Infections - microbiology
Feces
Feces - microbiology
Gene amplification
Genes
Health aspects
Humans
Laboratories
Medicine and Health Sciences
Methods
Molecular Diagnostic Techniques - instrumentation
Molecular Diagnostic Techniques - methods
Nucleic Acid Amplification Techniques - instrumentation
Nucleic Acid Amplification Techniques - methods
Pathogens
Research and analysis methods
Sample preparation
Samples
Sensitivity analysis
Shigella
Shigella - genetics
Shigella - isolation & purification
Shigella sonnei
Social Sciences
Specificity
Strains
Toxins
Tropical diseases
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Description
Summary:Enterotoxigenic E. coli (ETEC) and Shigella spp (Shigella) are complex pathogens. The diagnostic assays currently used to detect these pathogens are elaborate or complicated, which make them difficult to apply in resource poor settings where these diseases are endemic. The culture methods used to detect Shigella are not sensitive, and the methods used to detect ETEC are only available in a few research labs. To address this gap, we developed a rapid and simple diagnostic assay-"Rapid LAMP based Diagnostic Test (RLDT)." The six minutes sample preparation method directly from the fecal samples with lyophilized reaction strips and using established Loop-mediated Isothermal Amplification (LAMP) platform, ETEC [heat labile toxin (LT) and heat stable toxins (STh, and STp) genes] and Shigella (ipaH gene) detection was made simple, rapid (
ISSN:1935-2735
1935-2727
1935-2735
DOI:10.1371/journal.pntd.0010180