Effect of silver nanoparticles associated with fluoride on the progression of root dentin caries in vitro

• Published in: PloS one 2023-01, Vol.18 (1), p.e0277275
• Main Authors: Dias, Flaviana Alves, Vidal, Cristina M P, Comnick, Carissa L, Xie, Xian Jin, Berger, Sandrine Bittencourt
• Format: Article
• Language: English
• Subjects: Analysis; Biology and Life Sciences; Care and treatment; Cariostatic Agents; Collagen; Collagen - pharmacology; Collagenase; Collagenases - pharmacology; Collagens; Confocal microscopy; Cycles; Degradation; Demineralization; Demineralizing; Dental caries; Dental Caries - drug therapy; Dental Caries - pathology; Dental Caries - prevention & control; Dental Caries Susceptibility; Dentin; Development and progression; Diamines; Enamel; Engineering and Technology; Fluoride; Fluoride treatments; Fluorides; Fluorides, Topical - pharmacology; Gelatinase A; Health aspects; Humans; Hydroxyproline; Integrity; Kruskal-Wallis test; Lesions; Medicine and Health Sciences; Metal Nanoparticles; Methods; Microhardness; Nanoparticles; Older people; Permeability; Physical Sciences; Proteolysis; Quaternary Ammonium Compounds - pharmacology; Remineralization; Research and Analysis Methods; Root Caries - drug therapy; Root Caries - pathology; Scanning microscopy; Silicon carbide; Silver; Silver - pharmacology; Silver Compounds - pharmacology; Statistical analysis; Teeth; Variance analysis; Weight loss
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0277275

To assess the anti-proteolytic effect and potential to inhibit dentin root caries progression of a silver nanoparticle and fluoride solution (CNanoF) in comparison to silver diamine fluoride (SDF). 48 specimens of root dentin artificial caries lesion were treated with 38% SDF, CNanoF, CNano or F (n = 6 per group). Ph cycling with demineralization and remineralization solutions simulated caries lesion progression. In addition, specimens were incubated with or without bacterial collagenase in the remineralization solution to induce dentin proteolytic degradation. Dentin degradation was assessed by weight loss rate and hydroxyproline (Hyp) release. Changes in cross-sectional microhardness, and lesion permeability and collagen integrity as determined by confocal laser scanning microscopy indicated potential for further demineralization inhibition. The effect of the solutions on the activity of metalloproteinases (MMP) -2 and -9 was also investigated. Statistical analysis consisted of ANOVA, Kruskal-Wallis, and linear mixed models with post-hoc pairwise Tukey, Dunn, and t-tests (α = 0.05). Treatment with SDF resulted in lower weight loss rate than did other solutions, but all groups showed similar Hyp release (p = 0.183). SDF resulted in greater microhardness at superficial layers of the caries lesions (p0.05), with or without the use of collagenase (p = 0.58). No statistically significant difference was noted among solutions regarding collagen integrity after pH cycling; however, SDF-treated dentin had a significant decrease in collagen integrity when collagenase was used (p = 0.003). Interestingly, only SDF was able to completely inactivate MMP-2 and -9. CNanoF and SDF both potentially prevent dentin degradation during caries lesion progression in vitro; however, SDF was more effective at inhibiting further tissue demineralization.