Quantitative comparison of manuka and clover honey proteomes with royal jelly

Royal jelly and honey are two substances produced successively by the worker bee caste. Modern proteomics approaches have been used to explore the protein component of each substance independently, but to date none have quantitatively compared the protein profile of honey and royal jelly directly. S...

Full description

Saved in:
Bibliographic Details
Published in:PloS one 2023-02, Vol.18 (2), p.e0272898-e0272898
Main Authors: Paget, Blake W, Kleffmann, Torsten, Whiteman, Kim E, Thomas, Mark F, McMahon, Chris D
Format: Article
Language:English
Subjects:
Animals
Bees
Biology and Life Sciences
Clover
Comparative analysis
Evaluation
Fatty Acids - analysis
Hemodialysis
Honey
Honey - analysis
Mass spectrometry
Mass spectroscopy
Medicine and Health Sciences
Metabolism
Nectar
Peptides
Physiological aspects
Plant Nectar
Properties
Proteins
Proteome
Proteomes
Proteomics
Royal jelly
Scientific imaging
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Royal jelly and honey are two substances produced successively by the worker bee caste. Modern proteomics approaches have been used to explore the protein component of each substance independently, but to date none have quantitatively compared the protein profile of honey and royal jelly directly. Sequential window acquisition of all theoretical fragment-ion spectra mass spectrometry (SWATH-MS) was used to compare protein quantities of bee origin in mānuka and clover honey to royal jelly. Two analysis techniques identified 76 proteins in total. Peptide intensity was directly compared for a subset of 31 proteins that were identified with high confidence, and the relative changes in protein abundance were compared between each honey type and royal jelly. Major Royal Jelly Proteins (MRJPs) had similar profiles in both honeys, except MRJP6, which was significantly more abundant in clover honey. Proteins involved in nectar metabolism were more abundant in honey than in royal jelly as expected. However, the trend revealed a potential catalytic role for MRJP6 in clover honey and a nectar- or honey-specific role for uncharacterised protein LOC408608. The abundance of MRJP6 in mānuka honey was equivalent to royal jelly suggesting a potential effect of nectar type on expression of this protein. Data are available via ProteomeXchange with identifier PXD038889.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0272898