Fzr regulates silk gland growth by promoting endoreplication and protein synthesis in the silkworm

Silkworm silk gland cells undergo endoreplicating cycle and rapid growth during the larval period, and synthesize massive silk proteins for silk production. In this study, we demonstrated that a binary transgenic CRISPR/Cas9 approach-mediated Fzr mutation in silkworm posterior silk gland (PSG) cells...

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Bibliographic Details
Published in:PLoS genetics 2023-01, Vol.19 (1), p.e1010602-e1010602
Main Authors: Qian, Wenliang, Li, Hao, Zhang, Xing, Tang, Yaohao, Yuan, Dongqin, Huang, Zhu, Cheng, Daojun
Format: Article
Language:English
Subjects:
Animals
Biology and Life Sciences
Biosynthesis
Bombyx
Cell cycle
CRISPR
Cyclins - genetics
DNA biosynthesis
DNA replication
Endoreduplication
Evolution
Gene mutations
Genes
Genetic aspects
Genetic research
Insect Proteins - genetics
Insect Proteins - metabolism
Insects
mRNA
Mutation
Physiological aspects
Protein biosynthesis
Protein Biosynthesis - genetics
Protein expression
Protein synthesis
Proteins
Proteomes
Replication
Research and Analysis Methods
Silk
Silk - genetics
Silk gland
Silkworms
Ubiquitin
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Summary:Silkworm silk gland cells undergo endoreplicating cycle and rapid growth during the larval period, and synthesize massive silk proteins for silk production. In this study, we demonstrated that a binary transgenic CRISPR/Cas9 approach-mediated Fzr mutation in silkworm posterior silk gland (PSG) cells caused an arrest of silk gland growth and a decrease in silk production. Mechanistically, PSG-specific Fzr mutation blocked endoreplication progression by inducing an expression dysregulation of several cyclin proteins and DNA replication-related regulators. Moreover, based on label-free quantitative proteome analysis, we showed in PSG cells that Fzr mutation-induced decrease in the levels of cyclin proteins and silk proteins was likely due to an inhibition of the ribosome biogenesis pathway associated with mRNA translation, and/or an enhance of the ubiquitin-mediated protein degradation pathway. Rbin-1 inhibitor-mediated blocking of ribosomal biogenesis pathway decreased DNA replication in PSG cells and silk production. Altogether, our results reveal that Fzr positively regulates PSG growth and silk production in silkworm by promoting endoreplication and protein synthesis in PSG cells.
ISSN:1553-7404
1553-7390
1553-7404
DOI:10.1371/journal.pgen.1010602