RNA sequencing revealed the multi-stage transcriptome transformations during the development of gallbladder cancer associated with chronic inflammation

Gallbladder cancer (GBC) is a highly malignant tumor with extremely poor prognosis. Previous studies have suggested that the carcinogenesis and progression of GBC is a multi-stage and multi-step process, but most of them focused on the genome changes. And a few studies just compared the transcriptom...

Full description

Saved in:
Bibliographic Details
Published in:PloS one 2023-03, Vol.18 (3), p.e0283770-e0283770
Main Authors: Yang, Sen, Qin, Litao, Wu, Pan, Liu, Yanbing, Zhang, Yanling, Mao, Bing, Yan, Yiyang, Yan, Shuai, Tan, Feilong, Yue, Xueliang, Liu, Hongshan, Xue, Huanzhou
Format: Article
Language:English
Subjects:
Abnormalities
Analysis
Biology and life sciences
Calculi
Cancer
Cancer therapies
Carcinogenesis
Carcinogens
Cell Line, Tumor
Cell migration
Cluster analysis
Comparative analysis
Consent
Data analysis
Data collection
Development and progression
Diagnosis
Evolution
Experiments
Gallbladder
Gallbladder cancer
Gallbladder diseases
Gallbladder Neoplasms - pathology
Gallstones
Gene expression
Gene sequencing
Genetic aspects
Genomes
Genomics
Humans
Inflammation
Inflammation - genetics
Instrument industry
Lipid metabolism
Lipids
Medical prognosis
Medicine and Health Sciences
Membrane proteins
Metabolism
Non-coding RNA
Prognosis
Quality control
Research and analysis methods
RNA
RNA sequencing
RNA, Long Noncoding - genetics
Sequence Analysis, RNA
Sex hormones
Transcriptome
Transcriptomes
Tumors
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by cdi_FETCH-LOGICAL-c693t-3c900f354b292597e99757128bb8a80cc300fa1c6b4de79f0fb91ef12b3976913
cites cdi_FETCH-LOGICAL-c693t-3c900f354b292597e99757128bb8a80cc300fa1c6b4de79f0fb91ef12b3976913
container_end_page e0283770
container_issue 3
container_start_page e0283770
container_title PloS one
container_volume 18
creator Yang, Sen
Qin, Litao
Wu, Pan
Liu, Yanbing
Zhang, Yanling
Mao, Bing
Yan, Yiyang
Yan, Shuai
Tan, Feilong
Yue, Xueliang
Liu, Hongshan
Xue, Huanzhou
description Gallbladder cancer (GBC) is a highly malignant tumor with extremely poor prognosis. Previous studies have suggested that the carcinogenesis and progression of GBC is a multi-stage and multi-step process, but most of them focused on the genome changes. And a few studies just compared the transcriptome differences between tumor tissues and adjacent noncancerous tissues. The transcriptome changes, relating to every stage of GBC evolution, have rarely been studied. We selected three cases of normal gallbladder, four cases of gallbladder with chronic inflammation induced by gallstones, five cases of early GBC, and five cases of advanced GBC, using next-generation RNA sequencing to reveal the changes in mRNAs and lncRNAs expression during the evolution of GBC. In-depth analysis of the sequencing data indicated that transcriptome changes from normal gallbladder to gallbladder with chronic inflammation were distinctly related to inflammation, lipid metabolism, and sex hormone metabolism; transcriptome changes from gallbladder with chronic inflammation to early GBC were distinctly related to immune activities and connection between cells; and the transcriptome changes from early GBC to advanced GBC were distinctly related to transmembrane transport of substances and migration of cells. Expression profiles of mRNAs and lncRNAs change significantly during the evolution of GBC, in which lipid-based metabolic abnormalities play an important promotive role, inflammation and immune activities play a key role, and membrane proteins are very highlighted molecular changes.
doi_str_mv 10.1371/journal.pone.0283770
format article
fullrecord <record><control><sourceid>gale_plos_</sourceid><recordid>TN_cdi_plos_journals_2792881977</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A743645991</galeid><doaj_id>oai_doaj_org_article_0fad3d93f1e447298364d6640021455f</doaj_id><sourcerecordid>A743645991</sourcerecordid><originalsourceid>FETCH-LOGICAL-c693t-3c900f354b292597e99757128bb8a80cc300fa1c6b4de79f0fb91ef12b3976913</originalsourceid><addsrcrecordid>eNqNk9tu1DAQhiMEoqXwBggiISG42MWHxI6v0KrisFJFpXK4tRzHSbxy7K3tFHgSXhfvblptUC9QLpzY3_9PZsaTZc8hWEJM4buNG70VZrl1Vi0BqjCl4EF2ChlGC4IAfnj0fpI9CWEDQIkrQh5nJ5gwRlAJT7M_V19WeVDXo7JS2y736kYJo5o89iofRhP1IkTRqTx6YYP0ehvdMH21zg8iamdD3ox-p96JmuRg3HZQNuauzTthTG1E0yifS2FlWkQITmoRU5SfOva57L2zWubatkYMB8un2aNWmKCeTetZ9v3jh2_nnxcXl5_W56uLhSQMxwWWDIAWl0WNGCoZVYzRkkJU1XUlKiAlTscCSlIXjaKsBW3NoGohqjGjhEF8lr08-G6NC3yqaeCIMlRVkFGaiPWBaJzY8K3Xg_C_uROa7zec77jwUUujeArV4IbhFqqioIhVmBQNIQUACBZl2Sav91O0sR5UI1ONvDAz0_mJ1T3v3A2HABBEYJEc3kwO3qWmhcgHHaQyRljlxv2PY1YRuE_t1T_o_elNVGqU4qkFLgWWO1O-okVKoGR7r-U9VHoaNWiZbmCr0_5M8HYmSExUv2InxhD4-uvV_7OXP-bs6yO2T3c19sGZcX8N52BxAKV3IXjV3lUZAr4boNtq8N0A8WmAkuzFcYfuRLcTg_8CWgcYeA</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2792881977</pqid></control><display><type>article</type><title>RNA sequencing revealed the multi-stage transcriptome transformations during the development of gallbladder cancer associated with chronic inflammation</title><source>NCBI_PubMed Central（免费）</source><source>Publicly Available Content Database</source><creator>Yang, Sen ; Qin, Litao ; Wu, Pan ; Liu, Yanbing ; Zhang, Yanling ; Mao, Bing ; Yan, Yiyang ; Yan, Shuai ; Tan, Feilong ; Yue, Xueliang ; Liu, Hongshan ; Xue, Huanzhou</creator><contributor>Singh, Ajay Pratap</contributor><creatorcontrib>Yang, Sen ; Qin, Litao ; Wu, Pan ; Liu, Yanbing ; Zhang, Yanling ; Mao, Bing ; Yan, Yiyang ; Yan, Shuai ; Tan, Feilong ; Yue, Xueliang ; Liu, Hongshan ; Xue, Huanzhou ; Singh, Ajay Pratap</creatorcontrib><description>Gallbladder cancer (GBC) is a highly malignant tumor with extremely poor prognosis. Previous studies have suggested that the carcinogenesis and progression of GBC is a multi-stage and multi-step process, but most of them focused on the genome changes. And a few studies just compared the transcriptome differences between tumor tissues and adjacent noncancerous tissues. The transcriptome changes, relating to every stage of GBC evolution, have rarely been studied. We selected three cases of normal gallbladder, four cases of gallbladder with chronic inflammation induced by gallstones, five cases of early GBC, and five cases of advanced GBC, using next-generation RNA sequencing to reveal the changes in mRNAs and lncRNAs expression during the evolution of GBC. In-depth analysis of the sequencing data indicated that transcriptome changes from normal gallbladder to gallbladder with chronic inflammation were distinctly related to inflammation, lipid metabolism, and sex hormone metabolism; transcriptome changes from gallbladder with chronic inflammation to early GBC were distinctly related to immune activities and connection between cells; and the transcriptome changes from early GBC to advanced GBC were distinctly related to transmembrane transport of substances and migration of cells. Expression profiles of mRNAs and lncRNAs change significantly during the evolution of GBC, in which lipid-based metabolic abnormalities play an important promotive role, inflammation and immune activities play a key role, and membrane proteins are very highlighted molecular changes.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0283770</identifier><identifier>PMID: 36996251</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Abnormalities ; Analysis ; Biology and life sciences ; Calculi ; Cancer ; Cancer therapies ; Carcinogenesis ; Carcinogens ; Cell Line, Tumor ; Cell migration ; Cluster analysis ; Comparative analysis ; Consent ; Data analysis ; Data collection ; Development and progression ; Diagnosis ; Evolution ; Experiments ; Gallbladder ; Gallbladder cancer ; Gallbladder diseases ; Gallbladder Neoplasms - pathology ; Gallstones ; Gene expression ; Gene sequencing ; Genetic aspects ; Genomes ; Genomics ; Humans ; Inflammation ; Inflammation - genetics ; Instrument industry ; Lipid metabolism ; Lipids ; Medical prognosis ; Medicine and Health Sciences ; Membrane proteins ; Metabolism ; Non-coding RNA ; Prognosis ; Quality control ; Research and analysis methods ; RNA ; RNA sequencing ; RNA, Long Noncoding - genetics ; Sequence Analysis, RNA ; Sex hormones ; Transcriptome ; Transcriptomes ; Tumors</subject><ispartof>PloS one, 2023-03, Vol.18 (3), p.e0283770-e0283770</ispartof><rights>Copyright: © 2023 Yang et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.</rights><rights>COPYRIGHT 2023 Public Library of Science</rights><rights>2023 Yang et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2023 Yang et al 2023 Yang et al</rights><rights>2023 Yang et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c693t-3c900f354b292597e99757128bb8a80cc300fa1c6b4de79f0fb91ef12b3976913</citedby><cites>FETCH-LOGICAL-c693t-3c900f354b292597e99757128bb8a80cc300fa1c6b4de79f0fb91ef12b3976913</cites><orcidid>0000-0002-6243-9639</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.proquest.com/docview/2792881977/fulltextPDF?pq-origsite=primo$$EPDF$$P50$$Gproquest$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.proquest.com/docview/2792881977?pq-origsite=primo$$EHTML$$P50$$Gproquest$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,25753,27924,27925,37012,37013,44590,53791,53793,75126</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/36996251$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Singh, Ajay Pratap</contributor><creatorcontrib>Yang, Sen</creatorcontrib><creatorcontrib>Qin, Litao</creatorcontrib><creatorcontrib>Wu, Pan</creatorcontrib><creatorcontrib>Liu, Yanbing</creatorcontrib><creatorcontrib>Zhang, Yanling</creatorcontrib><creatorcontrib>Mao, Bing</creatorcontrib><creatorcontrib>Yan, Yiyang</creatorcontrib><creatorcontrib>Yan, Shuai</creatorcontrib><creatorcontrib>Tan, Feilong</creatorcontrib><creatorcontrib>Yue, Xueliang</creatorcontrib><creatorcontrib>Liu, Hongshan</creatorcontrib><creatorcontrib>Xue, Huanzhou</creatorcontrib><title>RNA sequencing revealed the multi-stage transcriptome transformations during the development of gallbladder cancer associated with chronic inflammation</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Gallbladder cancer (GBC) is a highly malignant tumor with extremely poor prognosis. Previous studies have suggested that the carcinogenesis and progression of GBC is a multi-stage and multi-step process, but most of them focused on the genome changes. And a few studies just compared the transcriptome differences between tumor tissues and adjacent noncancerous tissues. The transcriptome changes, relating to every stage of GBC evolution, have rarely been studied. We selected three cases of normal gallbladder, four cases of gallbladder with chronic inflammation induced by gallstones, five cases of early GBC, and five cases of advanced GBC, using next-generation RNA sequencing to reveal the changes in mRNAs and lncRNAs expression during the evolution of GBC. In-depth analysis of the sequencing data indicated that transcriptome changes from normal gallbladder to gallbladder with chronic inflammation were distinctly related to inflammation, lipid metabolism, and sex hormone metabolism; transcriptome changes from gallbladder with chronic inflammation to early GBC were distinctly related to immune activities and connection between cells; and the transcriptome changes from early GBC to advanced GBC were distinctly related to transmembrane transport of substances and migration of cells. Expression profiles of mRNAs and lncRNAs change significantly during the evolution of GBC, in which lipid-based metabolic abnormalities play an important promotive role, inflammation and immune activities play a key role, and membrane proteins are very highlighted molecular changes.</description><subject>Abnormalities</subject><subject>Analysis</subject><subject>Biology and life sciences</subject><subject>Calculi</subject><subject>Cancer</subject><subject>Cancer therapies</subject><subject>Carcinogenesis</subject><subject>Carcinogens</subject><subject>Cell Line, Tumor</subject><subject>Cell migration</subject><subject>Cluster analysis</subject><subject>Comparative analysis</subject><subject>Consent</subject><subject>Data analysis</subject><subject>Data collection</subject><subject>Development and progression</subject><subject>Diagnosis</subject><subject>Evolution</subject><subject>Experiments</subject><subject>Gallbladder</subject><subject>Gallbladder cancer</subject><subject>Gallbladder diseases</subject><subject>Gallbladder Neoplasms - pathology</subject><subject>Gallstones</subject><subject>Gene expression</subject><subject>Gene sequencing</subject><subject>Genetic aspects</subject><subject>Genomes</subject><subject>Genomics</subject><subject>Humans</subject><subject>Inflammation</subject><subject>Inflammation - genetics</subject><subject>Instrument industry</subject><subject>Lipid metabolism</subject><subject>Lipids</subject><subject>Medical prognosis</subject><subject>Medicine and Health Sciences</subject><subject>Membrane proteins</subject><subject>Metabolism</subject><subject>Non-coding RNA</subject><subject>Prognosis</subject><subject>Quality control</subject><subject>Research and analysis methods</subject><subject>RNA</subject><subject>RNA sequencing</subject><subject>RNA, Long Noncoding - genetics</subject><subject>Sequence Analysis, RNA</subject><subject>Sex hormones</subject><subject>Transcriptome</subject><subject>Transcriptomes</subject><subject>Tumors</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><sourceid>PIMPY</sourceid><sourceid>DOA</sourceid><recordid>eNqNk9tu1DAQhiMEoqXwBggiISG42MWHxI6v0KrisFJFpXK4tRzHSbxy7K3tFHgSXhfvblptUC9QLpzY3_9PZsaTZc8hWEJM4buNG70VZrl1Vi0BqjCl4EF2ChlGC4IAfnj0fpI9CWEDQIkrQh5nJ5gwRlAJT7M_V19WeVDXo7JS2y736kYJo5o89iofRhP1IkTRqTx6YYP0ehvdMH21zg8iamdD3ox-p96JmuRg3HZQNuauzTthTG1E0yifS2FlWkQITmoRU5SfOva57L2zWubatkYMB8un2aNWmKCeTetZ9v3jh2_nnxcXl5_W56uLhSQMxwWWDIAWl0WNGCoZVYzRkkJU1XUlKiAlTscCSlIXjaKsBW3NoGohqjGjhEF8lr08-G6NC3yqaeCIMlRVkFGaiPWBaJzY8K3Xg_C_uROa7zec77jwUUujeArV4IbhFqqioIhVmBQNIQUACBZl2Sav91O0sR5UI1ONvDAz0_mJ1T3v3A2HABBEYJEc3kwO3qWmhcgHHaQyRljlxv2PY1YRuE_t1T_o_elNVGqU4qkFLgWWO1O-okVKoGR7r-U9VHoaNWiZbmCr0_5M8HYmSExUv2InxhD4-uvV_7OXP-bs6yO2T3c19sGZcX8N52BxAKV3IXjV3lUZAr4boNtq8N0A8WmAkuzFcYfuRLcTg_8CWgcYeA</recordid><startdate>20230330</startdate><enddate>20230330</enddate><creator>Yang, Sen</creator><creator>Qin, Litao</creator><creator>Wu, Pan</creator><creator>Liu, Yanbing</creator><creator>Zhang, Yanling</creator><creator>Mao, Bing</creator><creator>Yan, Yiyang</creator><creator>Yan, Shuai</creator><creator>Tan, Feilong</creator><creator>Yue, Xueliang</creator><creator>Liu, Hongshan</creator><creator>Xue, Huanzhou</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope><orcidid>https://orcid.org/0000-0002-6243-9639</orcidid></search><sort><creationdate>20230330</creationdate><title>RNA sequencing revealed the multi-stage transcriptome transformations during the development of gallbladder cancer associated with chronic inflammation</title><author>Yang, Sen ; Qin, Litao ; Wu, Pan ; Liu, Yanbing ; Zhang, Yanling ; Mao, Bing ; Yan, Yiyang ; Yan, Shuai ; Tan, Feilong ; Yue, Xueliang ; Liu, Hongshan ; Xue, Huanzhou</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c693t-3c900f354b292597e99757128bb8a80cc300fa1c6b4de79f0fb91ef12b3976913</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>Abnormalities</topic><topic>Analysis</topic><topic>Biology and life sciences</topic><topic>Calculi</topic><topic>Cancer</topic><topic>Cancer therapies</topic><topic>Carcinogenesis</topic><topic>Carcinogens</topic><topic>Cell Line, Tumor</topic><topic>Cell migration</topic><topic>Cluster analysis</topic><topic>Comparative analysis</topic><topic>Consent</topic><topic>Data analysis</topic><topic>Data collection</topic><topic>Development and progression</topic><topic>Diagnosis</topic><topic>Evolution</topic><topic>Experiments</topic><topic>Gallbladder</topic><topic>Gallbladder cancer</topic><topic>Gallbladder diseases</topic><topic>Gallbladder Neoplasms - pathology</topic><topic>Gallstones</topic><topic>Gene expression</topic><topic>Gene sequencing</topic><topic>Genetic aspects</topic><topic>Genomes</topic><topic>Genomics</topic><topic>Humans</topic><topic>Inflammation</topic><topic>Inflammation - genetics</topic><topic>Instrument industry</topic><topic>Lipid metabolism</topic><topic>Lipids</topic><topic>Medical prognosis</topic><topic>Medicine and Health Sciences</topic><topic>Membrane proteins</topic><topic>Metabolism</topic><topic>Non-coding RNA</topic><topic>Prognosis</topic><topic>Quality control</topic><topic>Research and analysis methods</topic><topic>RNA</topic><topic>RNA sequencing</topic><topic>RNA, Long Noncoding - genetics</topic><topic>Sequence Analysis, RNA</topic><topic>Sex hormones</topic><topic>Transcriptome</topic><topic>Transcriptomes</topic><topic>Tumors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Yang, Sen</creatorcontrib><creatorcontrib>Qin, Litao</creatorcontrib><creatorcontrib>Wu, Pan</creatorcontrib><creatorcontrib>Liu, Yanbing</creatorcontrib><creatorcontrib>Zhang, Yanling</creatorcontrib><creatorcontrib>Mao, Bing</creatorcontrib><creatorcontrib>Yan, Yiyang</creatorcontrib><creatorcontrib>Yan, Shuai</creatorcontrib><creatorcontrib>Tan, Feilong</creatorcontrib><creatorcontrib>Yue, Xueliang</creatorcontrib><creatorcontrib>Liu, Hongshan</creatorcontrib><creatorcontrib>Xue, Huanzhou</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Gale_Opposing Viewpoints In Context</collection><collection>Science in Context</collection><collection>ProQuest Central (Corporate)</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Nursing &amp; Allied Health Database</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Meteorological &amp; Geoastrophysical Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>PHMC-Proquest健康医学期刊库</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science &amp; Engineering Collection</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central</collection><collection>Advanced Technologies &amp; Aerospace Collection</collection><collection>Agricultural &amp; Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health &amp; Medical Complete (Alumni)</collection><collection>Materials Science Database</collection><collection>Nursing &amp; Allied Health Database (Alumni Edition)</collection><collection>Meteorological &amp; Geoastrophysical Abstracts - Academic</collection><collection>ProQuest Engineering Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Agriculture Science Database</collection><collection>Health &amp; Medical Collection (Alumni Edition)</collection><collection>PML(ProQuest Medical Library)</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>ProQuest Biological Science Journals</collection><collection>Engineering Database</collection><collection>Nursing &amp; Allied Health Premium</collection><collection>Advanced Technologies &amp; Aerospace Database</collection><collection>ProQuest Advanced Technologies &amp; Aerospace Collection</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environmental Science Database</collection><collection>Materials science collection</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Engineering Collection</collection><collection>Environmental Science Collection</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yang, Sen</au><au>Qin, Litao</au><au>Wu, Pan</au><au>Liu, Yanbing</au><au>Zhang, Yanling</au><au>Mao, Bing</au><au>Yan, Yiyang</au><au>Yan, Shuai</au><au>Tan, Feilong</au><au>Yue, Xueliang</au><au>Liu, Hongshan</au><au>Xue, Huanzhou</au><au>Singh, Ajay Pratap</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>RNA sequencing revealed the multi-stage transcriptome transformations during the development of gallbladder cancer associated with chronic inflammation</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2023-03-30</date><risdate>2023</risdate><volume>18</volume><issue>3</issue><spage>e0283770</spage><epage>e0283770</epage><pages>e0283770-e0283770</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Gallbladder cancer (GBC) is a highly malignant tumor with extremely poor prognosis. Previous studies have suggested that the carcinogenesis and progression of GBC is a multi-stage and multi-step process, but most of them focused on the genome changes. And a few studies just compared the transcriptome differences between tumor tissues and adjacent noncancerous tissues. The transcriptome changes, relating to every stage of GBC evolution, have rarely been studied. We selected three cases of normal gallbladder, four cases of gallbladder with chronic inflammation induced by gallstones, five cases of early GBC, and five cases of advanced GBC, using next-generation RNA sequencing to reveal the changes in mRNAs and lncRNAs expression during the evolution of GBC. In-depth analysis of the sequencing data indicated that transcriptome changes from normal gallbladder to gallbladder with chronic inflammation were distinctly related to inflammation, lipid metabolism, and sex hormone metabolism; transcriptome changes from gallbladder with chronic inflammation to early GBC were distinctly related to immune activities and connection between cells; and the transcriptome changes from early GBC to advanced GBC were distinctly related to transmembrane transport of substances and migration of cells. Expression profiles of mRNAs and lncRNAs change significantly during the evolution of GBC, in which lipid-based metabolic abnormalities play an important promotive role, inflammation and immune activities play a key role, and membrane proteins are very highlighted molecular changes.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>36996251</pmid><doi>10.1371/journal.pone.0283770</doi><tpages>e0283770</tpages><orcidid>https://orcid.org/0000-0002-6243-9639</orcidid><oa>free_for_read</oa></addata></record>
fulltext fulltext
identifier ISSN: 1932-6203
ispartof PloS one, 2023-03, Vol.18 (3), p.e0283770-e0283770
issn 1932-6203
1932-6203
language eng
recordid cdi_plos_journals_2792881977
source NCBI_PubMed Central（免费）; Publicly Available Content Database
subjects Abnormalities
Analysis
Biology and life sciences
Calculi
Cancer
Cancer therapies
Carcinogenesis
Carcinogens
Cell Line, Tumor
Cell migration
Cluster analysis
Comparative analysis
Consent
Data analysis
Data collection
Development and progression
Diagnosis
Evolution
Experiments
Gallbladder
Gallbladder cancer
Gallbladder diseases
Gallbladder Neoplasms - pathology
Gallstones
Gene expression
Gene sequencing
Genetic aspects
Genomes
Genomics
Humans
Inflammation
Inflammation - genetics
Instrument industry
Lipid metabolism
Lipids
Medical prognosis
Medicine and Health Sciences
Membrane proteins
Metabolism
Non-coding RNA
Prognosis
Quality control
Research and analysis methods
RNA
RNA sequencing
RNA, Long Noncoding - genetics
Sequence Analysis, RNA
Sex hormones
Transcriptome
Transcriptomes
Tumors
title RNA sequencing revealed the multi-stage transcriptome transformations during the development of gallbladder cancer associated with chronic inflammation
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-24T03%3A28%3A02IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=RNA%20sequencing%20revealed%20the%20multi-stage%20transcriptome%20transformations%20during%20the%20development%20of%20gallbladder%20cancer%20associated%20with%20chronic%20inflammation&rft.jtitle=PloS%20one&rft.au=Yang,%20Sen&rft.date=2023-03-30&rft.volume=18&rft.issue=3&rft.spage=e0283770&rft.epage=e0283770&rft.pages=e0283770-e0283770&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0283770&rft_dat=%3Cgale_plos_%3EA743645991%3C/gale_plos_%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c693t-3c900f354b292597e99757128bb8a80cc300fa1c6b4de79f0fb91ef12b3976913%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=2792881977&rft_id=info:pmid/36996251&rft_galeid=A743645991&rfr_iscdi=true