Risk factor of elevated matrix metalloproteinase-3 gene expression in synovial fluid in knee osteoarthritis women

• Published in: PloS one 2023-03, Vol.18 (3), p.e0283831-e0283831
• Main Authors: Sulastri, Delmi, Arnadi, Arnadi, Afriwardi, Afriwardi, Desmawati, Desmawati, Amir, Arni, Irawati, Nuzulia, Yanis, Amel, Yusrawati, Yusrawati
• Format: Article
• Language: English
• Subjects: Age; Analysis; Arthritis; Biology and Life Sciences; Body fat; Cartilage; Cartilage diseases; Chi-square test; Cross-Sectional Studies; Cytokines; Demographic aspects; Deoxyribonucleic acid; Diagnosis; DNA; Enzyme-linked immunosorbent assay; Enzymes; Extracellular matrix; Female; Gene Expression; Gene polymorphism; Genetic aspects; Genetic factors; Health aspects; Health risks; Hospitals; Humans; IL-1β; Informed consent; Interleukin; Interleukin 1; Knee; Matrix metalloproteinase; Matrix Metalloproteinase 3 - genetics; Matrix Metalloproteinase 3 - metabolism; Matrix metalloproteinases; Medicine and Health Sciences; Menopause; Metalloproteinase; Nutrition; Nutritional status; Obesity; Older people; Orthopedics; Osteoarthritis; Osteoarthritis, Knee - genetics; Osteoarthritis, Knee - metabolism; Overweight; Pathogenesis; Pathophysiology; Polymorphism; Proteases; Risk analysis; Risk Factors; Statistical analysis; Stromelysin 1; Synovial fluid; Synovial Fluid - metabolism; Tumor Necrosis Factor-alpha - metabolism; Tumor necrosis factor-TNF; Tumor necrosis factor-α; Women; Womens health
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0283831

Metalloproteinases-3 (MMP3) are the main enzymes involved in cartilage degradation. Several genetic and non-genetic factors can increase the expression of MMP3 in patients with osteoarthritis (OA). This study aims to analyze the risk factors associated with the expression of the MMP3 gene rs679620 fluid synovial knee OA patients. A cross-sectional study was conducted at the orthopedic polyclinic Arifin Achmad Riau Province and Ibn Sina Hospital in Pekanbaru City. Ninety women who experienced knee OA were taken as samples by consecutive sampling and then signed the informed consent. Data were obtained through interviews using a questionnaire about characteristics, followed by weight and height measurements. Interleukin-1 β (IL-1β) and Tumor Necrosis Factor (TNF-α) were examined from the synovial fluid using the enzyme-linked immunosorbent assay (ELISA) method. The Metalloproteinases-3 (MMP3) gene polymorphism rs679620 was obtained from the DNA analysis of joint fluid results in the Biomedical Laboratory of the Faculty of Medicine, Andalas University. The data was processed computerized and then analyzed using the correlation Spearman-Rank, and chi-square tests. The results of statistical analysis are considered significant if the p-value is 0.05. The MMP3 rs679620 gene polymorphism of the mutant type was 88.9%, with the same proportion of AG and GG alleles (44.4%). Subjects aged ≥ 60 years were 53.3%, 85.6% did not work and 84.4% had menopause. The highest degree of OA was grade 2 (53.3%), most of whom had a risky nutritional status (84.4%). The median expression of the MMP3 rs679620 gene was 5.28 copies number. There is a significant relationship between MMP3 gene polymorphism rs679620, age, IL-1β, and TNF-α with MMP3 gene expression rs679620. There is no significant relationship between BMI, work status, and menopausal status with MMP3 gene expression rs679620. Conclusion. MMP3 gene polymorphism rs679620, age, levels of IL-1β and TNF-α are risk factors for increased MMP3 gene rs679620 expression in female knee OA.