VSTM1-v2 does not drive human Th17 cell differentiation: A replication study

Signal inhibitory receptor on leukocytes-1 (SIRL-1) is an immune inhibitory receptor expressed on human myeloid cells. We previously showed that dendritic cell (DC)-driven Th17 cell differentiation of human naive CD4+ T cells requires presence of neutrophils, which is inhibited by SIRL-1 ligation. V...

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Bibliographic Details
Published in:PloS one 2023-04, Vol.18 (4), p.e0284404-e0284404
Main Authors: von Richthofen, Helen J, Hafkamp, Florianne M J, van Haperen, Anouk, de Jong, Esther C, Meyaard, Linde
Format: Article
Language:English
Subjects:
Biology and Life Sciences
CD4 antigen
Cell Differentiation
Cells
Cytokines
Dendritic Cells
Differentiation (biology)
Evaluation
Flow cytometry
Granulocytes
Health aspects
Helper cells
Humans
Leukocytes
Leukocytes (neutrophilic)
Lymphocytes
Lymphocytes T
Medicine and Health Sciences
Myeloid cells
Neutrophils
Protein Isoforms
Receptors
Research and Analysis Methods
T cells
Th17 Cells
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Summary:Signal inhibitory receptor on leukocytes-1 (SIRL-1) is an immune inhibitory receptor expressed on human myeloid cells. We previously showed that dendritic cell (DC)-driven Th17 cell differentiation of human naive CD4+ T cells requires presence of neutrophils, which is inhibited by SIRL-1 ligation. VSTM1-v2 is a soluble isoform of SIRL-1, which was previously proposed to function as a Th17 polarizing cytokine. Here, we investigated the effect of VSTM1-v2 on DC-driven Th17 cell development. Neutrophils induced DC-driven Th17 cell differentiation, which was not enhanced by VSTM1-v2. Similarly, we found no effect of VSTM1-v2 on cytokine-driven Th17 cell development. Thus, our results do not support a role for VSTM1-v2 in Th17 cell differentiation.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0284404