Skin microbiome alterations in upper extremity secondary lymphedema

Lymphedema is a chronic condition that commonly occur from lymphatic injury following surgical resection of solid malignancies. While many studies have centered on the molecular and immune pathways that perpetuate lymphatic dysfunction, the role of the skin microbiome in lymphedema development remai...

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Bibliographic Details
Published in:PloS one 2023-05, Vol.18 (5), p.e0283609-e0283609
Main Authors: Campbell, Adana-Christine, Fei, Teng, Baik, Jung Eun, Park, Hyeung Ju, Shin, Jinyeon, Kuonqui, Kevin, Brown, Stav, Sarker, Ananta, Kataru, Raghu P, Mehrara, Babak J
Format: Article
Language:English
Subjects:
Analysis
Antibiotics
Bacteria
Biological diversity
Biology and Life Sciences
Breast cancer
Cellulitis
Chronic illnesses
Dermatitis
Diagnosis
Gene sequencing
Genetic aspects
Health aspects
Heterogeneity
Humans
Infections
Lymphatic Vessels - pathology
Lymphedema
Lymphedema - pathology
Malignancy
Mathematical models
Medicine and Health Sciences
Microbiomes
Microbiota (Symbiotic organisms)
Microorganisms
Neoplasms - pathology
Patients
Regression analysis
Review boards
Ribosomal RNA
RNA sequencing
rRNA 16S
Skin
Skin - pathology
Skin diseases
Social Sciences
Statistical analysis
Statistical models
Upper Extremity
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Summary:Lymphedema is a chronic condition that commonly occur from lymphatic injury following surgical resection of solid malignancies. While many studies have centered on the molecular and immune pathways that perpetuate lymphatic dysfunction, the role of the skin microbiome in lymphedema development remains unclear. In this study, skin swabs collected from normal and lymphedema forearms of 30 patients with unilateral upper extremity lymphedema were analyzed by 16S ribosomal RNA sequencing. Statistical models for microbiome data were utilized to correlate clinical variables with microbial profiles. Overall, 872 bacterial taxa were identified. There were no significant differences in microbial alpha diversity of the colonizing bacteria between normal and lymphedema skin samples (p = 0.25). Notably, for patients without a history of infection, a one-fold change in relative limb volume was significantly associated with a 0.58-unit increase in Bray-Curtis microbial distance between paired limbs (95%CI = 0.11,1.05, p = 0.02). Additionally, several genera, including Propionibacterium and Streptococcus, demonstrated high variability between paired samples. In summary, we demonstrate high compositional heterogeneity in the skin microbiome in upper extremity secondary lymphedema, supporting future studies into the role of host-microbe interactions on lymphedema pathophysiology.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0283609