miR-4482 and miR-3912 aim for 3'UTR of ERG mRNA in prostate cancer

Ets-related gene (ERG) is overexpressed as a fusion protein in prostate cancer. During metastasis, the pathological role of ERG is associated with cell proliferation, invasion, and angiogenesis. Here, we hypothesized that miRNAs regulate ERG expression through its 3'UTR. Several bioinformatics...

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Bibliographic Details
Published in:PloS one 2023-06, Vol.18 (6), p.e0286996-e0286996
Main Authors: Mumtaz, Sidra, Usman Rashid, Muhammad, Khan, Rizwan Ullah, Malkani, Naila
Format: Article
Language:English
Subjects:
3' Untranslated Regions - genetics
Angiogenesis
Apoptosis
Binding Sites
Bioinformatics
Biology and life sciences
Cell adhesion & migration
Cell culture
Cell migration
Cell proliferation
Cloning
Fusion protein
Gene expression
Genes
Genes, Regulator
Humans
Male
Medicine and Health Sciences
Metastases
MicroRNAs
miRNA
Overexpression
Plasmids
Prostate
Prostate cancer
Prostatic Neoplasms - genetics
Proteins
Reporter gene
Research and Analysis Methods
Therapeutic targets
Thyroid cancer
Transcriptional Regulator ERG - genetics
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Summary:Ets-related gene (ERG) is overexpressed as a fusion protein in prostate cancer. During metastasis, the pathological role of ERG is associated with cell proliferation, invasion, and angiogenesis. Here, we hypothesized that miRNAs regulate ERG expression through its 3'UTR. Several bioinformatics tools were used to identify miRNAs and their binding sites on 3'UTR of ERG. The selected miRNAs expression was analyzed in prostate cancer samples by qPCR. The miRNAs overexpression was induced in prostate cancer cells (VCaP) to analyze ERG expression. Reporter gene assay was performed to evaluate the ERG activity in response to selected miRNAs. The expression of ERG downstream target genes was also investigated through qPCR after miRNAs overexpression. To observe the effects of selected miRNAs on cell proliferation and migration, scratch assay was performed to calculate the cell migration rate. miR-4482 and miR-3912 were selected from bioinformatics databases. miR-4482 and -3912 expression were decreased in prostate cancer samples, as compared to controls (p
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0286996