Lipoxin receptor agonist and inhibition of LTA 4 hydrolase prevent tight junction disruption caused by P . aeruginosa filtrate in airway epithelial cells

Airway diseases can disrupt tight junction proteins, compromising the epithelial barrier and making it more permeable to pathogens. In people with pulmonary disease who are prone to infection with Pseudomonas aeruginosa , pro-inflammatory leukotrienes are increased and anti-inflammatory lipoxins are...

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Bibliographic Details
Published in:PloS one 2023-07, Vol.18 (7)
Main Authors: Kalsi, Kameljit, Jackson, Sonya, Baines, Deborah
Format: Article
Language:English
Subjects:
Agonists
Antibodies
Asthma
Bacterial infections
Cancer
Cell division
Cell junctions
Cell lines
Chronic obstructive pulmonary disease
Cloning
Cystic fibrosis
E-cadherin
Epithelial cells
Epithelium
Filtrate
Infections
Inflammation
Inhibitors
Interleukin 6
Interleukin 8
Leukotrienes
Lung diseases
Microscopy
Permeability
Proteins
Pseudomonas aeruginosa
Receptors
Respiratory tract diseases
Virulence
Zonula occludens-1 protein
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Summary:Airway diseases can disrupt tight junction proteins, compromising the epithelial barrier and making it more permeable to pathogens. In people with pulmonary disease who are prone to infection with Pseudomonas aeruginosa , pro-inflammatory leukotrienes are increased and anti-inflammatory lipoxins are decreased. Upregulation of lipoxins is effective in counteracting inflammation and infection. However, whether combining a lipoxin receptor agonist with a specific leukotriene A 4 hydrolase (LTA 4 H) inhibitor could enhance these protective effects has not to our knowledge been investigated. Therefore, we explored the effect of lipoxin receptor agonist BML-111 and JNJ26993135 a specific LTA 4 H inhibitor that prevents the production of pro-inflammatory LTB 4 on tight junction proteins disrupted by P . aeruginosa filtrate (PAF) in human airway epithelial cell lines H441 and 16HBE-14o. Pre-treatment with BML-111 prevented an increase in epithelial permeability induced by PAF and conserved ZO-1 and claudin-1 at the cell junctions. JNJ26993135 similarly prevented the increased permeability induced by PAF, restored ZO-1 and E-cadherin and reduced IL-8 but not IL-6. Cells pre-treated with BML-111 plus JNJ26993135 restored TEER and permeability, ZO-1 and claudin-1 to the cell junctions. Taken together, these data indicate that the combination of a lipoxin receptor agonist with a LTA 4 H inhibitor could provide a more potent therapy.
ISSN:1932-6203
DOI:10.1371/journal.pone.0287183