The role of photobehaviour in sponge larval dispersal and settlement

• Published in: PloS one 2023-07, Vol.18 (7), p.e0287989-e0287989
• Main Author: Whalan, Steve
• Format: Article
• Language: English
• Subjects: Analysis; Animals; Behavior; Behavior, Animal - physiology; Biology and Life Sciences; Cilia; Cincloramphus mathewsi; Dispersal; Ecology; Ecosystem; Environmental aspects; Environmental conditions; Fluorescence; Fluorescence microscopy; Habitat selection; Habitats; Haliclona; Health aspects; Invertebrates; Larva - physiology; Larvae; Light; Light attenuation; Light effects; Metamorphosis; Microscopy; Organisms; Photobiology; Photoreceptors; Phototaxis; Physical Sciences; Porifera; Proteins; Shallow water; Social Sciences; Species; Spectral signatures; Wavelengths; White light
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0287989

Deciphering the behavioural ecology of adult (sessile) sponges is challenging. However, their motile larval stages afford opportunities to investigate how behaviour contributes to dispersal and selection of habitat. Light is a fundamental cue contributing to larval sponge dispersal where photoreceptive cells contribute to this process. But how universal is light as a cue to sponge larval dispersal and settlement? Behavioural choice experiments were used to test the effect of light on dispersal and settlement behaviours. Larvae of the tropical sponge species Coscinoderma mathewsi, Luffariella variabilis, Ircinia microconnulosa, and Haliclona sp., from deep (12-15 m) and shallower-water habitats (2-5 m), were used in experiments. Dispersal experiments provided a light-gradient-choice where light represented light attenuation with depth. Light treatments included white light and the spectral components of red and blue light. Settlement experiments comprised a choice between illuminated and shaded treatments. Fluorescence microscopy was used to establish the presence of fluorescent proteins associated with posterior locomotory cilia. Deeper-water species, C. mathewsi and I. microconnulosa discriminate light spectral signatures. Both species changed dispersal behaviour to light spectra as larvae aged. For C. mathewsi positive phototaxis to blue light changed to photophobic responses (all light treatments) after six hours and behaviours in I. microconnulosa changed from positive to negative phototaxis (white light) after six hours. L. variabilis, also a deeper-water species, was negatively phototactic to all light treatments. Larvae from the shallow-water species, Haliclona sp., moved towards all light wavelengths tested. There was no effect of light on settlement of the shallow-water Haliclona sp., but larvae in all three deeper-water species showed significantly higher settlement in shaded treatments. Fluorescence microscopy showed discrete fluorescent bands contiguous to posterior tufted cilia in all four species. These fluorescent bands may play a contributory role in larval photobehaviour.