Evaluation of Xpert MTB/RIF assay for the diagnosis of extrapulmonary tuberculosis in Southwest China

The purpose of this study was to determine the diagnostic efficacy of Xpert MTB/RIF assay for rapid diagnosis of Tuberculosis (TB) and detection of rifampicin (RIF) resistance in patients suspected of having EPTB, assessing it against traditional culture and drug susceptibility test (DST) by proport...

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Bibliographic Details
Published in:PLoS neglected tropical diseases 2023-06, Vol.17 (6), p.e0011403-e0011403
Main Authors: Li, Tong-Xin, Wang, Jing, Yang, Yu-Sheng, Wang, Peng-Sen, Zhou, Gang, Liao, Chuan-Yu, Zhang, Hui-Zheng, Luo, Ming, Zeng, Xiao-Gang, Yang, Guo-Qiang, Yang, Li-Jun, Chen, Yao-Kai
Format: Article
Language:English
Subjects:
Abscesses
Acids
Agreements
Ascites
Assaying
Automation
Biology and Life Sciences
Cerebrospinal fluid
Detection
Diagnosis
Dosage and administration
Drug resistance
Drug resistance in microorganisms
Drug therapy
Drugs
Ethics
Laboratories
Lymphatic system
Medicine and Health Sciences
Microscopy
Multidrug resistance
Mycobacterium tuberculosis
Pleural effusion
Prevention
Public health
Rifampin
Sensitivity
Specificity
Statistical analysis
Thorax
Tropical diseases
Tuberculosis
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Summary:The purpose of this study was to determine the diagnostic efficacy of Xpert MTB/RIF assay for rapid diagnosis of Tuberculosis (TB) and detection of rifampicin (RIF) resistance in patients suspected of having EPTB, assessing it against traditional culture and drug susceptibility test (DST) by proportional method, and the ability to predict multidrug resistance TB by Xpert MTB/RIF assay. In this study, the Xpert MTB/RIF assay was applied to 1,614 extrapulmonary specimens. Compared with TB culture and Composite Reference Standard (CRS), the Xpert MTB/RIF assay had a high sensitivity and specificity for detection of EPTB. Depending on the culture method or CRS as the standard, sensitivity of the Xpert MTB/RIF assay for detection of MTB in pleural effusion, cerebrospinal fluid, thoracic drainage fluid and throat swabs specimens were lower than that of other specimens. According to the experimental results, we have reason to believe that Xpert MTB/RIF assay is a rapid and simple technique with high sensitivity and specificity for diagnosing EPTB and detecting drug resistance in variety of specimens. Xpert MTB/RIF assay combined with DST maybe identify more cases of multi-drug resistant tuberculosis (MDR-TB).
ISSN:1935-2735
1935-2727
1935-2735
DOI:10.1371/journal.pntd.0011403