A biologist's guide to planning and performing quantitative bioimaging experiments

Technological advancements in biology and microscopy have empowered a transition from bioimaging as an observational method to a quantitative one. However, as biologists are adopting quantitative bioimaging and these experiments become more complex, researchers need additional expertise to carry out...

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Bibliographic Details
Published in:PLoS biology 2023-06, Vol.21 (6), p.e3002167-e3002167
Main Authors: Senft, Rebecca A, Diaz-Rohrer, Barbara, Colarusso, Pina, Swift, Lucy, Jamali, Nasim, Jambor, Helena, Pengo, Thomas, Brideau, Craig, Llopis, Paula Montero, Uhlmann, Virginie, Kirk, Jason, Gonzales, Kevin Andrew, Bankhead, Peter, Evans, 3rd, Edward L, Eliceiri, Kevin W, Cimini, Beth A
Format: Article
Language:English
Subjects:
Best practice
Biological research
Biologists
Biology
Biology, Experimental
Computer and Information Sciences
Data interpretation
Decision making
Engineering and Technology
Experiments
Image acquisition
Image analysis
Image processing
Image Processing, Computer-Assisted
Light
Measurement techniques
Medical imaging
Methods
Microscope and microscopy
Microscopy
Observational method
People and Places
Physical Sciences
Research and Analysis Methods
Sample preparation
Science Policy
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Summary:Technological advancements in biology and microscopy have empowered a transition from bioimaging as an observational method to a quantitative one. However, as biologists are adopting quantitative bioimaging and these experiments become more complex, researchers need additional expertise to carry out this work in a rigorous and reproducible manner. This Essay provides a navigational guide for experimental biologists to aid understanding of quantitative bioimaging from sample preparation through to image acquisition, image analysis, and data interpretation. We discuss the interconnectedness of these steps, and for each, we provide general recommendations, key questions to consider, and links to high-quality open-access resources for further learning. This synthesis of information will empower biologists to plan and execute rigorous quantitative bioimaging experiments efficiently.
ISSN:1545-7885
1544-9173
1545-7885
DOI:10.1371/journal.pbio.3002167