Energy-saving pretreatments affect pelagic Sargassum composition and DNA metabarcoding reveals the microbial community involved in methane yield

Sargassum spp. flood the Caribbean coastline, causing damage to the local economy and environment. Anaerobic digestion (AD) has been proposed as an attractive option for turning macroalgae into valuable resources. Sargassum spp. has a complex composition that affects the microbial composition involv...

Full description

Saved in:
Bibliographic Details
Published in:PloS one 2023-08, Vol.18 (8), p.e0289972-e0289972
Main Authors: Salgado-Hernández, Enrique, Ortiz-Ceballos, Ángel Isauro, Alvarado-Lassman, Alejandro, Martínez-Hernández, Sergio, Rosas-Mendoza, Erik Samuel, Velázquez-Fernández, Jesús Bernardino, Dorantes-Acosta, Ana Elena
Format: Article
Language:English
Subjects:
Advertising executives
Algae
Anaerobic digestion
Analysis
Animals
Archaea
Biodiesel fuels
Biofuels
Biogas
Biology and Life Sciences
Biomass
Coastal flooding
Community involvement
Community structure
Composition
Deoxyribonucleic acid
DNA
DNA barcoding
DNA Barcoding, Taxonomic
Ecology and Environmental Sciences
Energy
Energy conservation
Energy management systems
Energy use
Engineering and Technology
Flood damage
Genetic research
Inorganic compounds
Local economy
Methane
Methane production
Methanosarcina
Microbial activity
Microbiomes
Microbiota - genetics
Microorganisms
Next-generation sequencing
Physical Sciences
Plastics
Pretreatment
Sargassum
Seaweeds
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by cdi_FETCH-LOGICAL-c627t-2412b7ef2b4f85114ec5be016ec6f0ccfcff41c3c1cb13df4d0030b486fd3af3
cites cdi_FETCH-LOGICAL-c627t-2412b7ef2b4f85114ec5be016ec6f0ccfcff41c3c1cb13df4d0030b486fd3af3
container_end_page e0289972
container_issue 8
container_start_page e0289972
container_title PloS one
container_volume 18
creator Salgado-Hernández, Enrique
Ortiz-Ceballos, Ángel Isauro
Alvarado-Lassman, Alejandro
Martínez-Hernández, Sergio
Rosas-Mendoza, Erik Samuel
Velázquez-Fernández, Jesús Bernardino
Dorantes-Acosta, Ana Elena
description Sargassum spp. flood the Caribbean coastline, causing damage to the local economy and environment. Anaerobic digestion (AD) has been proposed as an attractive option for turning macroalgae into valuable resources. Sargassum spp. has a complex composition that affects the microbial composition involved in AD which generates a low methane yield. This study aimed to improve the methane yield of pelagic Sargassum, using different energy-saving pretreatments and identifying the microbial community associated with methane production. We applied different energy-saving pretreatments to algal biomass and assessed the methane yield using a biomethane potential (BMP) test. The microbial communities involved in the AD of the best- and worst-performing methanogenic systems were analyzed by high-throughput sequencing. The results showed that pretreatment modified the content of inorganic compounds, fibers, and the C:N ratio, which had a strong positive correlation with BMP. The water washing pretreatment resulted in the best methane yield, with an increase of 38%. DNA metabarcoding analysis revealed that the bacterial genera Marinilabiliaceae_uncultured, DMER64, Treponema, and Hydrogenispora, as well as the archaea genera Methanosarcina, RumEn_M2, Bathyarchaeia, and Methanomassiliicocus, dominated the microbial community with a high methane yield. This study is the first to demonstrate the microbial community structure involved in the AD of Sargassum spp. The pretreatments presented in this study can help overcome the limitations associated with methane yield.
doi_str_mv 10.1371/journal.pone.0289972
format article
fullrecord <record><control><sourceid>gale_plos_</sourceid><recordid>TN_cdi_plos_journals_2852599502</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A761278802</galeid><sourcerecordid>A761278802</sourcerecordid><originalsourceid>FETCH-LOGICAL-c627t-2412b7ef2b4f85114ec5be016ec6f0ccfcff41c3c1cb13df4d0030b486fd3af3</originalsourceid><addsrcrecordid>eNqNkl1rFDEUhgdRbF39B6IDgujFrvmYzytZatVCsWCLtyGTOZlNySTTJLO4_8KfbKadll3pheQiIXne9yQnb5K8xmiFaYk_XdvRGa5XgzWwQqSq65I8SY5xTcmyIIg-3VsfJS-8v0Yop1VRPE-OaJnXiCB0nPw5NeC63dLzrTJdOjgIDnjowQSfcilBhHQAzTsl0kvuOu792KfC9oP1KihrUm7a9MuPddpD4A13wraTkYMtcO3TsIG0V8LZRnE96frRqLBLldlavYU2LiblhhtIdwp0-zJ5JqMQXs3zIrn6enp18n15fvHt7GR9vhQFKcOSZJg0JUjSZLLKMc5A5A0gXIAoJBJCCikzLKjAosG0lVmLEEVNVhWypVzSRfL2znbQ1rO5l56RKid5XeeIROLzTIxND62IHXFcs8Gpnrsds1yxwxOjNqyzW4ZRRrMaTw4fZgdnb0bwgfXKC9A6PtaOt8VondE83myRvPsHffxKM9VxDUwZaWNhMZmydVlgUlbVLbV6hIqjhfgTMS1Sxf0DwccDQWQC_A4dH71nZ5c__5-9-HXIvt9jNzEPYeOtHqfY-EMwuwNjTrx3IB-6jBGbwn7fDTaFnc1hj7I3-z_0ILpPN_0Lbob-Jg</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2852599502</pqid></control><display><type>article</type><title>Energy-saving pretreatments affect pelagic Sargassum composition and DNA metabarcoding reveals the microbial community involved in methane yield</title><source>PubMed Central</source><source>ProQuest Publicly Available Content database</source><creator>Salgado-Hernández, Enrique ; Ortiz-Ceballos, Ángel Isauro ; Alvarado-Lassman, Alejandro ; Martínez-Hernández, Sergio ; Rosas-Mendoza, Erik Samuel ; Velázquez-Fernández, Jesús Bernardino ; Dorantes-Acosta, Ana Elena</creator><contributor>Li, Huan</contributor><creatorcontrib>Salgado-Hernández, Enrique ; Ortiz-Ceballos, Ángel Isauro ; Alvarado-Lassman, Alejandro ; Martínez-Hernández, Sergio ; Rosas-Mendoza, Erik Samuel ; Velázquez-Fernández, Jesús Bernardino ; Dorantes-Acosta, Ana Elena ; Li, Huan</creatorcontrib><description>Sargassum spp. flood the Caribbean coastline, causing damage to the local economy and environment. Anaerobic digestion (AD) has been proposed as an attractive option for turning macroalgae into valuable resources. Sargassum spp. has a complex composition that affects the microbial composition involved in AD which generates a low methane yield. This study aimed to improve the methane yield of pelagic Sargassum, using different energy-saving pretreatments and identifying the microbial community associated with methane production. We applied different energy-saving pretreatments to algal biomass and assessed the methane yield using a biomethane potential (BMP) test. The microbial communities involved in the AD of the best- and worst-performing methanogenic systems were analyzed by high-throughput sequencing. The results showed that pretreatment modified the content of inorganic compounds, fibers, and the C:N ratio, which had a strong positive correlation with BMP. The water washing pretreatment resulted in the best methane yield, with an increase of 38%. DNA metabarcoding analysis revealed that the bacterial genera Marinilabiliaceae_uncultured, DMER64, Treponema, and Hydrogenispora, as well as the archaea genera Methanosarcina, RumEn_M2, Bathyarchaeia, and Methanomassiliicocus, dominated the microbial community with a high methane yield. This study is the first to demonstrate the microbial community structure involved in the AD of Sargassum spp. The pretreatments presented in this study can help overcome the limitations associated with methane yield.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0289972</identifier><identifier>PMID: 37590200</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Advertising executives ; Algae ; Anaerobic digestion ; Analysis ; Animals ; Archaea ; Biodiesel fuels ; Biofuels ; Biogas ; Biology and Life Sciences ; Biomass ; Coastal flooding ; Community involvement ; Community structure ; Composition ; Deoxyribonucleic acid ; DNA ; DNA barcoding ; DNA Barcoding, Taxonomic ; Ecology and Environmental Sciences ; Energy ; Energy conservation ; Energy management systems ; Energy use ; Engineering and Technology ; Flood damage ; Genetic research ; Inorganic compounds ; Local economy ; Methane ; Methane production ; Methanosarcina ; Microbial activity ; Microbiomes ; Microbiota - genetics ; Microorganisms ; Next-generation sequencing ; Physical Sciences ; Plastics ; Pretreatment ; Sargassum ; Seaweeds</subject><ispartof>PloS one, 2023-08, Vol.18 (8), p.e0289972-e0289972</ispartof><rights>Copyright: © 2023 Salgado-Hernández et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.</rights><rights>COPYRIGHT 2023 Public Library of Science</rights><rights>2023 Salgado-Hernández et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2023 Salgado-Hernández et al 2023 Salgado-Hernández et al</rights><rights>2023 Salgado-Hernández et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c627t-2412b7ef2b4f85114ec5be016ec6f0ccfcff41c3c1cb13df4d0030b486fd3af3</citedby><cites>FETCH-LOGICAL-c627t-2412b7ef2b4f85114ec5be016ec6f0ccfcff41c3c1cb13df4d0030b486fd3af3</cites><orcidid>0000-0001-8700-4503 ; 0000-0003-3578-9913 ; 0000-0003-0611-8758</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.proquest.com/docview/2852599502/fulltextPDF?pq-origsite=primo$$EPDF$$P50$$Gproquest$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.proquest.com/docview/2852599502?pq-origsite=primo$$EHTML$$P50$$Gproquest$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,25753,27924,27925,37012,37013,44590,53791,53793,75126</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/37590200$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Li, Huan</contributor><creatorcontrib>Salgado-Hernández, Enrique</creatorcontrib><creatorcontrib>Ortiz-Ceballos, Ángel Isauro</creatorcontrib><creatorcontrib>Alvarado-Lassman, Alejandro</creatorcontrib><creatorcontrib>Martínez-Hernández, Sergio</creatorcontrib><creatorcontrib>Rosas-Mendoza, Erik Samuel</creatorcontrib><creatorcontrib>Velázquez-Fernández, Jesús Bernardino</creatorcontrib><creatorcontrib>Dorantes-Acosta, Ana Elena</creatorcontrib><title>Energy-saving pretreatments affect pelagic Sargassum composition and DNA metabarcoding reveals the microbial community involved in methane yield</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Sargassum spp. flood the Caribbean coastline, causing damage to the local economy and environment. Anaerobic digestion (AD) has been proposed as an attractive option for turning macroalgae into valuable resources. Sargassum spp. has a complex composition that affects the microbial composition involved in AD which generates a low methane yield. This study aimed to improve the methane yield of pelagic Sargassum, using different energy-saving pretreatments and identifying the microbial community associated with methane production. We applied different energy-saving pretreatments to algal biomass and assessed the methane yield using a biomethane potential (BMP) test. The microbial communities involved in the AD of the best- and worst-performing methanogenic systems were analyzed by high-throughput sequencing. The results showed that pretreatment modified the content of inorganic compounds, fibers, and the C:N ratio, which had a strong positive correlation with BMP. The water washing pretreatment resulted in the best methane yield, with an increase of 38%. DNA metabarcoding analysis revealed that the bacterial genera Marinilabiliaceae_uncultured, DMER64, Treponema, and Hydrogenispora, as well as the archaea genera Methanosarcina, RumEn_M2, Bathyarchaeia, and Methanomassiliicocus, dominated the microbial community with a high methane yield. This study is the first to demonstrate the microbial community structure involved in the AD of Sargassum spp. The pretreatments presented in this study can help overcome the limitations associated with methane yield.</description><subject>Advertising executives</subject><subject>Algae</subject><subject>Anaerobic digestion</subject><subject>Analysis</subject><subject>Animals</subject><subject>Archaea</subject><subject>Biodiesel fuels</subject><subject>Biofuels</subject><subject>Biogas</subject><subject>Biology and Life Sciences</subject><subject>Biomass</subject><subject>Coastal flooding</subject><subject>Community involvement</subject><subject>Community structure</subject><subject>Composition</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>DNA barcoding</subject><subject>DNA Barcoding, Taxonomic</subject><subject>Ecology and Environmental Sciences</subject><subject>Energy</subject><subject>Energy conservation</subject><subject>Energy management systems</subject><subject>Energy use</subject><subject>Engineering and Technology</subject><subject>Flood damage</subject><subject>Genetic research</subject><subject>Inorganic compounds</subject><subject>Local economy</subject><subject>Methane</subject><subject>Methane production</subject><subject>Methanosarcina</subject><subject>Microbial activity</subject><subject>Microbiomes</subject><subject>Microbiota - genetics</subject><subject>Microorganisms</subject><subject>Next-generation sequencing</subject><subject>Physical Sciences</subject><subject>Plastics</subject><subject>Pretreatment</subject><subject>Sargassum</subject><subject>Seaweeds</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><sourceid>PIMPY</sourceid><recordid>eNqNkl1rFDEUhgdRbF39B6IDgujFrvmYzytZatVCsWCLtyGTOZlNySTTJLO4_8KfbKadll3pheQiIXne9yQnb5K8xmiFaYk_XdvRGa5XgzWwQqSq65I8SY5xTcmyIIg-3VsfJS-8v0Yop1VRPE-OaJnXiCB0nPw5NeC63dLzrTJdOjgIDnjowQSfcilBhHQAzTsl0kvuOu792KfC9oP1KihrUm7a9MuPddpD4A13wraTkYMtcO3TsIG0V8LZRnE96frRqLBLldlavYU2LiblhhtIdwp0-zJ5JqMQXs3zIrn6enp18n15fvHt7GR9vhQFKcOSZJg0JUjSZLLKMc5A5A0gXIAoJBJCCikzLKjAosG0lVmLEEVNVhWypVzSRfL2znbQ1rO5l56RKid5XeeIROLzTIxND62IHXFcs8Gpnrsds1yxwxOjNqyzW4ZRRrMaTw4fZgdnb0bwgfXKC9A6PtaOt8VondE83myRvPsHffxKM9VxDUwZaWNhMZmydVlgUlbVLbV6hIqjhfgTMS1Sxf0DwccDQWQC_A4dH71nZ5c__5-9-HXIvt9jNzEPYeOtHqfY-EMwuwNjTrx3IB-6jBGbwn7fDTaFnc1hj7I3-z_0ILpPN_0Lbob-Jg</recordid><startdate>20230817</startdate><enddate>20230817</enddate><creator>Salgado-Hernández, Enrique</creator><creator>Ortiz-Ceballos, Ángel Isauro</creator><creator>Alvarado-Lassman, Alejandro</creator><creator>Martínez-Hernández, Sergio</creator><creator>Rosas-Mendoza, Erik Samuel</creator><creator>Velázquez-Fernández, Jesús Bernardino</creator><creator>Dorantes-Acosta, Ana Elena</creator><general>Public Library of Science</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0001-8700-4503</orcidid><orcidid>https://orcid.org/0000-0003-3578-9913</orcidid><orcidid>https://orcid.org/0000-0003-0611-8758</orcidid></search><sort><creationdate>20230817</creationdate><title>Energy-saving pretreatments affect pelagic Sargassum composition and DNA metabarcoding reveals the microbial community involved in methane yield</title><author>Salgado-Hernández, Enrique ; Ortiz-Ceballos, Ángel Isauro ; Alvarado-Lassman, Alejandro ; Martínez-Hernández, Sergio ; Rosas-Mendoza, Erik Samuel ; Velázquez-Fernández, Jesús Bernardino ; Dorantes-Acosta, Ana Elena</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c627t-2412b7ef2b4f85114ec5be016ec6f0ccfcff41c3c1cb13df4d0030b486fd3af3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>Advertising executives</topic><topic>Algae</topic><topic>Anaerobic digestion</topic><topic>Analysis</topic><topic>Animals</topic><topic>Archaea</topic><topic>Biodiesel fuels</topic><topic>Biofuels</topic><topic>Biogas</topic><topic>Biology and Life Sciences</topic><topic>Biomass</topic><topic>Coastal flooding</topic><topic>Community involvement</topic><topic>Community structure</topic><topic>Composition</topic><topic>Deoxyribonucleic acid</topic><topic>DNA</topic><topic>DNA barcoding</topic><topic>DNA Barcoding, Taxonomic</topic><topic>Ecology and Environmental Sciences</topic><topic>Energy</topic><topic>Energy conservation</topic><topic>Energy management systems</topic><topic>Energy use</topic><topic>Engineering and Technology</topic><topic>Flood damage</topic><topic>Genetic research</topic><topic>Inorganic compounds</topic><topic>Local economy</topic><topic>Methane</topic><topic>Methane production</topic><topic>Methanosarcina</topic><topic>Microbial activity</topic><topic>Microbiomes</topic><topic>Microbiota - genetics</topic><topic>Microorganisms</topic><topic>Next-generation sequencing</topic><topic>Physical Sciences</topic><topic>Plastics</topic><topic>Pretreatment</topic><topic>Sargassum</topic><topic>Seaweeds</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Salgado-Hernández, Enrique</creatorcontrib><creatorcontrib>Ortiz-Ceballos, Ángel Isauro</creatorcontrib><creatorcontrib>Alvarado-Lassman, Alejandro</creatorcontrib><creatorcontrib>Martínez-Hernández, Sergio</creatorcontrib><creatorcontrib>Rosas-Mendoza, Erik Samuel</creatorcontrib><creatorcontrib>Velázquez-Fernández, Jesús Bernardino</creatorcontrib><creatorcontrib>Dorantes-Acosta, Ana Elena</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Gale In Context: Opposing Viewpoints database</collection><collection>Gale In Context: Science</collection><collection>ProQuest Central (Corporate)</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>ProQuest Nursing and Allied Health Source</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Meteorological &amp; Geoastrophysical Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health &amp; Medical Collection (Proquest)</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science &amp; Engineering Collection</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central UK/Ireland</collection><collection>Advanced Technologies &amp; Aerospace Collection</collection><collection>Agricultural &amp; Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health &amp; Medical Complete (Alumni)</collection><collection>Materials Science Database</collection><collection>Nursing &amp; Allied Health Database (Alumni Edition)</collection><collection>Meteorological &amp; Geoastrophysical Abstracts - Academic</collection><collection>ProQuest Engineering Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Agriculture Science Database</collection><collection>Health &amp; Medical Collection (Alumni Edition)</collection><collection>PML(ProQuest Medical Library)</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>ProQuest Biological Science Journals</collection><collection>Engineering Database</collection><collection>Nursing &amp; Allied Health Premium</collection><collection>ProQuest advanced technologies &amp; aerospace journals</collection><collection>ProQuest Advanced Technologies &amp; Aerospace Collection</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environmental Science Database</collection><collection>Materials science collection</collection><collection>ProQuest Publicly Available Content database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Engineering collection</collection><collection>Environmental Science Collection</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Salgado-Hernández, Enrique</au><au>Ortiz-Ceballos, Ángel Isauro</au><au>Alvarado-Lassman, Alejandro</au><au>Martínez-Hernández, Sergio</au><au>Rosas-Mendoza, Erik Samuel</au><au>Velázquez-Fernández, Jesús Bernardino</au><au>Dorantes-Acosta, Ana Elena</au><au>Li, Huan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Energy-saving pretreatments affect pelagic Sargassum composition and DNA metabarcoding reveals the microbial community involved in methane yield</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2023-08-17</date><risdate>2023</risdate><volume>18</volume><issue>8</issue><spage>e0289972</spage><epage>e0289972</epage><pages>e0289972-e0289972</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Sargassum spp. flood the Caribbean coastline, causing damage to the local economy and environment. Anaerobic digestion (AD) has been proposed as an attractive option for turning macroalgae into valuable resources. Sargassum spp. has a complex composition that affects the microbial composition involved in AD which generates a low methane yield. This study aimed to improve the methane yield of pelagic Sargassum, using different energy-saving pretreatments and identifying the microbial community associated with methane production. We applied different energy-saving pretreatments to algal biomass and assessed the methane yield using a biomethane potential (BMP) test. The microbial communities involved in the AD of the best- and worst-performing methanogenic systems were analyzed by high-throughput sequencing. The results showed that pretreatment modified the content of inorganic compounds, fibers, and the C:N ratio, which had a strong positive correlation with BMP. The water washing pretreatment resulted in the best methane yield, with an increase of 38%. DNA metabarcoding analysis revealed that the bacterial genera Marinilabiliaceae_uncultured, DMER64, Treponema, and Hydrogenispora, as well as the archaea genera Methanosarcina, RumEn_M2, Bathyarchaeia, and Methanomassiliicocus, dominated the microbial community with a high methane yield. This study is the first to demonstrate the microbial community structure involved in the AD of Sargassum spp. The pretreatments presented in this study can help overcome the limitations associated with methane yield.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>37590200</pmid><doi>10.1371/journal.pone.0289972</doi><tpages>e0289972</tpages><orcidid>https://orcid.org/0000-0001-8700-4503</orcidid><orcidid>https://orcid.org/0000-0003-3578-9913</orcidid><orcidid>https://orcid.org/0000-0003-0611-8758</orcidid><oa>free_for_read</oa></addata></record>
fulltext fulltext
identifier ISSN: 1932-6203
ispartof PloS one, 2023-08, Vol.18 (8), p.e0289972-e0289972
issn 1932-6203
1932-6203
language eng
recordid cdi_plos_journals_2852599502
source PubMed Central; ProQuest Publicly Available Content database
subjects Advertising executives
Algae
Anaerobic digestion
Analysis
Animals
Archaea
Biodiesel fuels
Biofuels
Biogas
Biology and Life Sciences
Biomass
Coastal flooding
Community involvement
Community structure
Composition
Deoxyribonucleic acid
DNA
DNA barcoding
DNA Barcoding, Taxonomic
Ecology and Environmental Sciences
Energy
Energy conservation
Energy management systems
Energy use
Engineering and Technology
Flood damage
Genetic research
Inorganic compounds
Local economy
Methane
Methane production
Methanosarcina
Microbial activity
Microbiomes
Microbiota - genetics
Microorganisms
Next-generation sequencing
Physical Sciences
Plastics
Pretreatment
Sargassum
Seaweeds
title Energy-saving pretreatments affect pelagic Sargassum composition and DNA metabarcoding reveals the microbial community involved in methane yield
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-25T02%3A02%3A23IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Energy-saving%20pretreatments%20affect%20pelagic%20Sargassum%20composition%20and%20DNA%20metabarcoding%20reveals%20the%20microbial%20community%20involved%20in%20methane%20yield&rft.jtitle=PloS%20one&rft.au=Salgado-Hern%C3%A1ndez,%20Enrique&rft.date=2023-08-17&rft.volume=18&rft.issue=8&rft.spage=e0289972&rft.epage=e0289972&rft.pages=e0289972-e0289972&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0289972&rft_dat=%3Cgale_plos_%3EA761278802%3C/gale_plos_%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c627t-2412b7ef2b4f85114ec5be016ec6f0ccfcff41c3c1cb13df4d0030b486fd3af3%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=2852599502&rft_id=info:pmid/37590200&rft_galeid=A761278802&rfr_iscdi=true