A highly antigenic fragment within the zoonotic Cryptosporidium parvum Gp900 glycoprotein (Domain 3) is absent in human restricted Cryptosporidium species

We identified a fragment (Domain 3—D3) of the immunodominant sporozoite surface glycoprotein of the zoonotic parasite Cryptosporidium gp900, which is absent C . hominis and C . parvum anthroponosum . The fragment is highly antigenic and is able to effectively differentiate between zoonotic C . parvu...

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Published in:PloS one 2023-08, Vol.18 (8), p.e0287997-e0287997
Main Authors: Dayao, Denise Ann E, Jaskiewicz, Justyna J, Sheoran, Abhineet S, Widmer, Giovanni, Tzipori, Saul
Format: Article
Language:English
Subjects:
Antigens
Biology and Life Sciences
Care and treatment
Children
Cryptosporidiosis
Cryptosporidium
Cryptosporidium hominis
Diagnosis
Diseases
E coli
Genotypes
Glycoproteins
Immunization
Infections
Laboratory animals
Medicine and Health Sciences
Parasites
Proteins
Protozoa
Research and Analysis Methods
Zoonoses
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creator Dayao, Denise Ann E
Jaskiewicz, Justyna J
Sheoran, Abhineet S
Widmer, Giovanni
Tzipori, Saul
description We identified a fragment (Domain 3—D3) of the immunodominant sporozoite surface glycoprotein of the zoonotic parasite Cryptosporidium gp900, which is absent C . hominis and C . parvum anthroponosum . The fragment is highly antigenic and is able to effectively differentiate between zoonotic C . parvum and species/genotypes that infect preferentially humans. D3 detection provides a serological tool to determine whether the source of human cryptosporidiosis is of animal or human origin. We demonstrate this in experimentally challenged piglets, mice, rats, and alpaca. We speculate that the absence of this fragment from the C . hominis and C . parvum anthroponosum gp900 protein may play a key role in their host restriction.
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subjects Antigens
Biology and Life Sciences
Care and treatment
Children
Cryptosporidiosis
Cryptosporidium
Cryptosporidium hominis
Diagnosis
Diseases
E coli
Genotypes
Glycoproteins
Immunization
Infections
Laboratory animals
Medicine and Health Sciences
Parasites
Proteins
Protozoa
Research and Analysis Methods
Zoonoses
title A highly antigenic fragment within the zoonotic Cryptosporidium parvum Gp900 glycoprotein (Domain 3) is absent in human restricted Cryptosporidium species
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