Cloning, expression, and molecular modification of glycoside hydrolase family 5 genes from Thermoascus aurantiacus

In this paper, a novel bifunctional cellulase gene cel1 was cloned from Thermoascus aurantiacus by PCR and heterologously expressed in Pichia pastoris GS115. Bioinformatics and other related tools were used to compare the nucleotide homology of target genes, and analyze the signal peptide, transmemb...

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Bibliographic Details
Published in:PloS one 2023-09, Vol.18 (9), p.e0285680-e0285680
Main Author: Shao, Hongwei
Format: Article
Language:English
Subjects:
Amino acids
Analysis
Bifunctional enzymes
Bioinformatics
Biology and Life Sciences
Cellulase
Cellulose
Cloning
Enzymes
Fungi
Gene expression
Genes
Genetic aspects
Glucose
Glucosidase
Glycosidases
Glycoside hydrolase
Homology
Hydrolases
Mutagenesis
Nucleotide sequence
Nucleotides
Plasmids
Properties
Protein structure
Proteins
Research and Analysis Methods
Tertiary structure
Thermoascus aurantiacus
Yeast
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Summary:In this paper, a novel bifunctional cellulase gene cel1 was cloned from Thermoascus aurantiacus by PCR and heterologously expressed in Pichia pastoris GS115. Bioinformatics and other related tools were used to compare the nucleotide homology of target genes, and analyze the signal peptide, transmembrane domain, hydrophilicity, secondary and tertiary structure of proteins. It was concluded that cel1 has similar endoglucanase nucleotide sequences and falls under the GH5 family. It was also found that cel1 has nucleotide sequences similar to glucosidase, which can infer that cel1 may have the properties of glucosidase, indicating that cel1 is multifunctional. At the same time, a part of the nucleotide sequence of the gene was removed to obtain a new gene cel2, and after highly efficient heterologous expression, its specific activity was found to be 2.1 times higher. Its enhancement is related to the exposure of the protein's hollow three-dimensional structure. This paper provides good material for exploring the relationship between the structure of bifunctional enzymes and their functions, which lays a solid foundation for further research and applications, and provides useful insight for gene mining of other novel enzymes.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0285680